Devin Scannell(1), Mario Fares(2), Ken Wolfe(1). (1)
Genetics Department, Trinity College Dublin, College Green, Dublin,
D2, Ireland; (2) Biology Department, N.U.I. Maynooth, Maynooth , Co.
We developed tools to identify sets of orthologous genes, and used
them to find groups of (co-) orthologous transcription factors from
ten currently available yeast genome sequences. These yeast
orthology groups (YOGs) were analyzed using likelihood and parsimony
based bioinformatics methods, to determine the selective constraints
operating in different lineages. In particular we test the
hypothesis that a massive and sudden increase in gene complement,
such as occurs in a whole genome duplication, should be accompanied
by complementary evolutionary changes in genes that encode
regulators of gene expression. Prior evidence for this comes from S.
cerevisiae where many gene pairs formed by polyploidy (ohnologs)
exist as aerobic and hypoxic pairs. We used a high quality set of
cis-regulatory elements (assembled by Kellis et al. based on
phylogenetic footprinting) to find cis-elements that are
over-represented in duplicated genes formed by polyploidy. The
association between these cis-elements and particular transcription
factors is investigated.
Program Nr. 517A from 2004 Yeast meeting