Presentation/Session Information

Session Information

Session Title: Cell Division and Cell Death Session Type: Parallel
Session Location: Northwest Auditorium Session Time: Thu, Jun 25 8:30AM - 11:30AM

Presentation Information

Program Number: 52 Presentation Time: 9:18AM - 9:30AM

Presentation Content

Telomere maintenance through recruitment of internal genomic regions.Beomseok Seo 1, Chuna Kim 1, Mark Hills 2, Sanghyun Sung 1, Hyesook Kim 1, Eunkyeong Kim 1, Daisy S. Lim 1, Hyun-Seok Oh 3, Rachael Mi Jung Choi 3, Jongsik Chun 3, Jaegal Shim 4, Junho Lee 1,5. 1)Department of Biological Sciences, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, Korea; 2)Terry Fox Laboratory, BC Cancer Agency, Vancouver V5Z 1L3, Canada; 3)Department of Biological Sciences, Bioinformatics Institute, BIO-MAX, Seoul National University, Seoul, Korea; 4)Research Institute, National Cancer Center, Goyang, Gyeonggi, Korea; 5)Department of Biophysics and Chemical Biology, Seoul National University, Seoul, Korea

 Telomere is a ribonucleoprotein complex that protects the natural ends of the linear chromosome. Telomere length is mainly maintained by telomerase, a reverse transcriptase that adds telomere repeat sequence. However recombination-dependent mechanism for telomere maintenance also exist in yeast and human cancer cells. In Caenorhabditis elegans, some of telomerase deletion mutant, trt-1(ok410), can maintain the telomere by alternative lengthening of telomeres (ALT). However the molecular mechanism of ALT in worms is largely unknown. In this study, we established stably maintained ALT survivors of worms that activate an ALT mechanism to escape from the sterility phenotype caused by telomerase deletion. By whole genome sequencing and  mapping we concluded that mutation may not be required to maintain ALT telomere in these survivors. Interestingly, ALT survivors added either of two specific internal genomic regions as templates for ALT(T-ALT) according to their genetic backgrounds. A T-ALT sequence consists of telomere-like repeat sequence with unrelated sequence in between. T-ALTs had already been copied to a proximal telomere region of the same chromosome prior to ALT activation in nature, and were amplified interchromosomally by ALT activation. Here we propose a hypothesis that the existence of T-ALT structure in the proximal telomere may be one of the prerequisites for ALT activation..

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