Presentation/Session Information

Session Information

Session Title: Epigenetics and Gene Regulation Session Type: Parallel
Session Location: Carnesale Palisades Ballroom Session Time: Thu, Jun 25 8:30AM - 11:30AM

Presentation Information

Program Number: 20 Presentation Time: 11:06AM - 11:18AM

Presentation Content

The C. elegans KDM5 homolog RBR-2 promotes cell fate acquisition by modifying H3K4 methylation levels at regulatory elements.Y.C. Lussi 1, L. Mariani 1, T.R. Myers 1, C. Krag 1, G. Wong 2, A.E. Salcini 1. 1)BRIC, University of Copenhagen, Copenhagen, Denmark; 2)A.I. Virtanen Institute, University of Eastern Finland, Kuopio, Finland

Methylation levels of histone 3 lysine 4 (H3K4) play a crucial role in transcription regulation and are finely controlled by the action of specific histone methyltransferases and demethylases during development. Here, we address the role of the H3K4me2/3 demethylase RBR-2, the unique member of the KDM5 class of histone demethylases in C. elegans, in cell differentiation during postembryonic development. We found that the enzymatic activity of RBR-2 is required cell-autonomously to regulate the 2° cell fate acquisition in vulva precursor cells and to achieve correct vulva formation. Genome-wide analysis indicates that RBR-2 is needed for the regulation of H3K4me levels in regulatory regions of the genome, including transcription start sites and enhancer regions. Loss of RBR-2 results not only in aberrant acquisition of H3K4me3 at transcription start sites, but also in loss of enhancer signatures in regulatory regions. Accordingly, RNA sequencing analysis shows both up- and down-regulation of gene transcription, suggesting a complex function of RBR-2 in regulating gene activity.

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