Presentation/Session Information

Session Information

Session Title: Epigenetics and Gene Regulation Session Type: Parallel
Session Location: Carnesale Palisades Ballroom Session Time: Thu, Jun 25 8:30AM - 11:30AM

Presentation Information

Program Number: 16 Presentation Time: 10:18AM - 10:30AM

Presentation Content

Condensin-Driven Remodeling of X-Chromosome Topology during Dosage Compensation.E. Crane 1,3,4, Q. Bian 1,4, R. McCord 2,4, B. Lajoie 2,4, B. Wheeler 1, E. Anderson 1, J. Dekker 2, B. Meyer 1. 1)Howard Hughes Medical Institute and University of California-Berkeley, Berkeley, CA; 2)University of Massachusetts Medical School, Worcester, MA; 3)Current address: Stanford University School of Medicine, Stanford, CA; 4)These authors contributed equally.

The three-dimensional organization of a genome plays a critical role in regulating gene expression, yet little is known about the machinery and mechanisms that determine higher-order chromosome structure. Here we perform genome-wide chromosome conformation capture analysis, FISH, and RNA-seq to obtain comprehensive 3D maps of the Caenorhabditis elegans genome and to dissect X-chromosome dosage compensation, which balances gene expression between XX hermaphrodites and XO males. The dosage compensation complex (DCC), a condensin complex, binds to both hermaphrodite X chromosomes via sequence-specific recruitment elements on X (rex sites) to reduce chromosome-wide gene expression by half. Most DCC condensin subunits also act in other condensin complexes to control the compaction and resolution of all mitotic and meiotic chromosomes. By comparing chromosome structure in wild-type and DCC-defective embryos, we show that the DCC remodels hermaphrodite X chromosomes into a sex-specific spatial conformation distinct from autosomes. Dosage-compensated X chromosomes consist of self-interacting domains (~1 Mb) resembling mammalian Topologically Associating Domains (TADs). TADs on X have stronger boundaries and more regular spacing than on autosomes. Many TAD boundaries on X coincide with the highest-affinity rex sites and become diminished or lost in DCC-defective mutants, thereby converting the topology of X to a conformation resembling autosomes. rex sites engage in DCC-dependent long-range interactions, with the most frequent interactions occurring between rex sites at DCC-dependent TAD boundaries. These results imply that the DCC reshapes the topology of X by forming new TAD boundaries and reinforcing weak boundaries through interactions between its highest-affinity binding sites. As this model predicts, deletion of endogenous rex sites at DCC-dependent TAD boundaries using CRISPR/Cas9 greatly diminishes the boundaries. Thus, the DCC imposes a distinct higher-order structure onto X while regulating gene expression chromosome wide..

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