Presentation/Session Information

Session Information

Session Title: Behavior Session Type: Parallel
Session Location: De Neve Auditorium Session Time: Thu, Jun 25 8:30AM - 11:30AM

Presentation Information

Program Number: 40 Presentation Time: 9:30AM - 9:42AM

Presentation Content

Pan-neuronal imaging in roaming animals.Vivek Venkatachalam 1,2, Ni Ji 1,2, Xian Wang 1,2, James Mitchell 1,2, Mason Klein 1,2, Christopher Tabone 1,2, Christopher Clark 3, Joel Greenwood 2, Andrew Chisholm 4, Jagan Srinivasan 5, Mark Alkema 3, Mei Zhen 6, Aravinthan Samuel 1,2. 1)Physics, Harvard University, Cambridge, MA; 2)Center for Brain Science, Harvard University, Cambridge, MA; 3)Neurobiology, University of Massachusetts Medical School, Worcester, MA; 4)Neurobiology, UCSD, La Jolla, CA; 5)Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA; 6)Molecular Genetics and Physiology, University of Toronto, Toronto, ON, Canada

We present an imaging system for panneuronal recording in crawling C. elegans. A spinning disk confocal microscope, modified for automated tracking of the C. elegans head ganglia, simultaneously records the activity and position of ~100 neurons that co-express cytoplasmic calcium indicator GCaMP6 and nuclear localized RFP at 10 volumes per second. We developed a behavioral analysis algorithm that maps the movements of the head ganglia to the animal’s posture and locomotion. Image registration and analysis software automatically assigns an index to each nucleus and calculates the corresponding calcium signal. Neurons with highly stereotyped positions can be associated with unique indexes and subsequently identified using an atlas of the worm nervous system. To test our system, we analyzed the brainwide activity patterns of worms subjected to thermosensory inputs. We uncover a strikingly sparse representation of thermosensory perception involving two neurons (AFDL and AFDR), and a broadly distributed representation of the motor state across the nervous system. Our imaging setup and analysis pipeline, panneuronal imaging in roaming animals, or PANERA, provides a new platform to establish functional maps of sensory to motor transformation in behaving C. elegans and Drosophila larva.




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