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Session Listing

 

 


Wednesday, June 24   10:30 AM–4:00 PM

GSA Trainee Boot Camp

Room:Southbay
Organizer:Beth Ruedi, Genetics Society of America

Wednesday, June 24   2:00 PM–5:00 PM

Parasitic Nematodes: Bridging the Divide Workshop

Room:Northwest Auditorium
Organizer:Marty Chalfie, Columbia University

Each year infections of animals and plants by parasitic nematodes cause many billions of dollars of agricultural damage. Over a billion people worldwide, particularly in developing nations, are infected by nematodes and suffer from the resulting debilitating diseases. Currently, only a few investigators address problems of parasitic nematodes using C. elegans. To encourage and facilitate more interactions between the C. elegans and parasitic nematode communities, a workshop was held at the 2013 International C. elegans Meeting in which experts in plant, animal and human parasitic nematodes spoke on the life history and unique biology of these parasitic species and on outstanding issues in their field. A key goal of this session was to make C. elegans scientists aware of the issues and problems that parasitic nematode researchers face and pave the way for applying the powerful approaches and technologies that have advanced C. elegans research to parasitic nematodes.

2:00 - 2:15pm      Andrew R. Burns, Genna M. Luciani, Gabriel Musso, Rachel Bagg, May Yeo, Yuqian Zhang, Luckshika Rajendran, John Glavin, Robert Hunter, Elizabeth Redman, Susan Stasiuk, Michael Schertzberg, Sean R. Cutler, Mike Tyers, Guri Giaever, Corey Nislow, Andrew G. Fraser, Calum A. MacRae, John Gilleard, and Peter J. Roy Caenorhabditis elegans is a useful model for anthelmintic discovery.

2:15 - 2:30pm      Mostafa Zamanian and Erik C. Andersen Elucidating conserved genetic mechanisms of anthelmintic resistance using Caenorhabditis nematodes.

2:30 - 2:45pm      Erich M. Schwarz, Yan Hu, Igor Antoshechkin, Melanie M. Miller, Paul W. Sternberg, Raffi V. Aroian The genome of Ancylostoma ceylanicum: distinguishing possible immunological decoys from possible drug and vaccine targets in a model hookworm.

2:45 - 3:00pm      Jillian Sesar, Yan Hu, Thanh-thanh Nguyen, David Koch and Raffi V. Aroian How we used C. elegans and hookworms to optimize an anthelmintic.

3:00 - 3:15pm      Janis C. Weeks, William M. Roberts, Kristin J. Robinson, Melissa Keaney, Jon J. Vermeire, Joseph F. Urban, Shawn R. Lockery, John Hawdon A microfluidic screening platform using electrophysiological recordings from parasitic larval stages of hookworm (Ancylostoma ceylanicum) and roundworm (Ascaris suum).

3:15 - 3:30pm      Hala Zahreddine Fahs, Robert White, Fathima Shaffra Refai, Patricia G. Cipriani, Fabio Piano, and Kristin C. Gunsalus High Throughput Chemical Genomics in C. elegans and P. pacificus: Applications for discovering new anthelmintics and their targets.

3:30 - 3:45pm      BREAK

3:45 - 4:00pm      Peter Hunt, Shilpa Kapoor, Stephen Doyle, and Warwick Grant The role of amphid signalling in ivermectin-mediated inhibition of reproduction in parasitic nematodes.

4:00 - 4:15pm      Delia O’Rourke, Dave Stroud and Jonathan Hodgkin Using bacterial pathogens to explore the nematode surface coat.

4:15 - 4:30pm      Emily M. Larsen, Laura J. Davies, Phuong T. Y. Dinh, Axel A. Elling, and Jennifer L. Watts Pilot studies to determine if dietary polyunsaturated fatty acids cause sterility in plant-parasitic nematodes.

4:30 - 4:45pm      Peter DiGennaro, Benjamin Bobay, Denis Fourchet, Charles H. Opperman, Dahlia Nielsen, Valerie Williamson, and David Bird Meloidogyne hapla: a robust model for genetic and structural analyses of parasitism.

4:45 - 5:00pm      Trisha Brock, Thomas Marshall, Mark Shenderovich, Ashok Bajai, Adriane Wolstenholme, and Chris Hopkins A Platform for Anthelmintic Drug Discovery using Genome-modified C. elegans.



Wednesday, June 24   4:00 PM–6:30 PM

Preparing your Educational Resources for Online Publication

Room:West Coast
Organizer:Beth Ruedi, Genetics Society of America

New for 2015! Educators who have been wondering how to prepare their teaching resources for publication in an online repository should apply for this workshop, which will guide attendees through the submission process for both CourseSource and GSA PREP, and give them a dedicated time to work on their submissions. Robin Wright (Univ of Minnesota), Editor-in-Chief for CourseSource and GSA Education Committee member, and Beth Ruedi (GSA) will be on hand to describe the two resource portals, answer questions, and provide guidance to attendees.



Wednesday, June 24   7:00 PM–9:00 PM
Royce Hall

Welcome and Opening Remarks
Benjamin Podbilewicz, Technion-IIT
Gillian Stanfield, University of Utah
GSA Welcome
Adam Fagen, GSA Executive Director
Plenary Session 1


Chair: Meera Sundaram, University of Pennsylvania

1- 7:10  From synapse to nucleus: the awesome power of worm genetics. Yishi Jin, University of California, San Diego, HHMI.

2- 7:40  The Mystery Cells of the Male: a novel pair of head interneurons required for sex differences in learning. Michele Sammut, Steven J. Cook, Ken Nguyen, Terry Felton, David H. Hall, Scott W. Emmons, Richard J. Poole, Arantza Barrios.

3- 7:52  Her Brain, His Behavior: Dimorphic neuronal connectivity and behavior are established by sex-specific synapse pruning during development. Meital Oren-Suissa, Oliver Hobert.

8:04 - Break

Chair: Oliver Hobert, Columbia University

4- 8:35  Caenorhabditis Genetics Center. Aric Daul, Theresa Stiernagle, Julie Knott, Kemi Awoyinka, Ann E. Rougvie.

5- 8:40  WormBook News. Jane Mendel, Qinghua Wang, Todd Harris, Paul Sternberg, Oliver Hobert, Martin Chalfie.

6- 8:45  WormBase 2015. Paul Sternberg, Kevin Howe, Paul Kersey, Matt Berriman, Todd Harris, Lincoln Stein, Tim Schedl, WormBase Consortium.

7- 8:50  Comprehensive Biology: How do we complete the C. elegans Knockout Project. Mark Edgley, Vinci Au, Katsufumi Dejima, Lisa Fernando, Stephane Flibotte, Sayaka Hori, Satoru Iwata, Angela Miller, Tomoko Motohashi, Greta Raymant, Yuji Suehiro, Jon Taylor, Sawako Yoshina, Shohei Mitani, Donald Moerman.

8- 8:55  What's new with WormAtlas? C. A. Wolkow, L. A. Herndon, Z. F. Altun, K. Fisher, C. Crocker, D. H. Hall.


Wednesday, June 24   9:00 PM–10:00 PM
Royce Hall

Historical Perspective: Celebration of the 20th International C. elegans Meeting


Chairs: Julie Ahringer, University of Cambridge and Paul Sternberg, HHMI and CalTech


Thursday, June 25   8:30 AM–11:30 AM
Carnesale Palisades Ballroom

Epigenetics and Gene Regulation


Chair: Oded Rechavi, Tel Aviv University

9- 8:30  nhr-33 encodes a nuclear receptor that is regulated by high zinc and mediates transcriptional activation to maintain zinc homeostasis in C. elegans. Kurt Warnhoff, Daniel Schneider, Zuzana Kocsisova, Chieh-Hsiang Tan, Hyun Roh, Andrew Morrison, Damari Croswell, Kerry Kornfeld.

10- 8:42  Intestinal bHLH factors regulate expression of a state-dependent chemoreceptor in ADL. Matt Gruner, Jeremy Grubbs, Dom Valdes.

11- 8:54  A novel method for time- and cell-specific RNAi reveals the profile of Let-60Ras in exploratory behavior. M. Hamakawa, T. Uozumi, N. Ueda, Y. Iino, T. Hirotsu.

12- 9:06  A C. elegans gene-centered protein-DNA interaction network uncovers functions for uncharacterized transcription factors and target genes. Juan I. Fuxman Bass, Carles Pons, John S. Reece-Hoyes, Shaleen Shrestha, Lucie Kozlowski, Akihiro Mori, Chad L. Myers, Albertha J. M. Walhout.

13- 9:18  Transcriptome analysis of the sex-specific differences in the somatic gonadal precursor cells in Caenorhabditis elegans. Mary B. Kroetz, David Zarkower.

14- 9:30  Genome-wide and species-wide variation in C. elegans reveals association of telomere length with population differences in pot-2. D. E. Cook, R. E. Tanny, D. Riccardi, L. Noble, M. V. Rockman, L. Kruglyak, E. C. Andersen.

9:42 - Break

Chair: Alla Grishok, Columbia University

15- 10:06  Deciphering the mechanism of X-upregulation in C. elegans dosage compensation. Alyssa Lau, Kevin Zhu, Gyorgyi Csankovszki.

16- 10:18  Condensin-Driven Remodeling of X-Chromosome Topology during Dosage Compensation. E. Crane, Q. Bian, R. McCord, B. Lajoie, B. Wheeler, E. Anderson, J. Dekker, B. Meyer.

17- 10:30  H3K9 methylation at repetitive elements safeguards genome integrity. P. Zeller, J. Padeken, S. Gasser.

18- 10:42  Epigenetic program of DNA replication. Ehsan Pourkarimi, James Bellush, Iestyn Whitehouse.

19- 10:54  Epigenetic memory in C. elegans: Plastic and elastic behaviors of histone modifications in response to rapid environmental changes. I. Celen, J. Doh, C. Sabanayagam.

20- 11:06  The C. elegans KDM5 homolog RBR-2 promotes cell fate acquisition by modifying H3K4 methylation levels at regulatory elements. Y. C. Lussi, L. Mariani, T. R. Myers, C. Krag, G. Wong, A. E. Salcini.

21- 11:18  Genome organization revealed through chromatin state mapping. Kenneth J. Evans, Przemyslaw Stempor, Michael A. Chesney, Thomas A. Down, Julie Ahringer.


Thursday, June 25   8:30 AM–11:30 AM
Grand Horizon Ballroom

Physiology: Aging and Stress I


Chair: Amir Sapir, University of Haifa at Oranim

22- 8:30  Synthetic superviability: combining detrimental mutations can have unexpected lifespan enhancing consequences. Hayley Lees, Lynne Cox, Alison Woollard.

23- 8:42  Autophagy promotes heat resistance and intestinal biomass conversion in ageing hermaphrodites. Alexandre Benedetto, Marina Ezcurra, Ann Gilliat, Filipe Cabreiro, Catherine Au, Jennifer Tullet, David Gems.

24- 8:54  Autophagy-mediated longevity is modulated by lipoprotein biogenesis. C. Daniel De Magalhaes Filho, Nicole Seah, Anna Petrashen, Hope R. Henderson, Jade Laguer, Julissa Gonzalez, Andy Dillin, Malene Hansen, Louis R. Lapierre.

25- 9:06  A massive increase in lipid and protein content in aging hermaphrodites. Marina Ezcurra, Catherine Au, Thanet Sornda, Alexandre Benedetto, Ann Gilliat, David Gems.

26- 9:18  MML-1/Mondo complexes regulate HLH-30/TFEB via TOR inhibition to promote longevity in response to signals from the reproductive system. S. Nakamura, O. Karalay, P. Jaeger, M. Horikawa, K. Nakamura, C. Latza, C. Klein, S. Templer, C. Dieterich, A. Antebi.

27- 9:30  SBP-1 and MDT-15 moderate the life-shortening effect of glucose by promoting fat conversion. Dongyeop Lee, Dae-Eun Joeng, Heehwa G. Son, Yasuyo Yamaoka, Hyunmin Kim, Keunhee Seo, Abdul Aziz Khan, Tae-Young Roh, Dae Won Moon, Youngsook Lee, Seung-Jae V. Lee.

9:42 - Break

Chair: Eyleen O'Rourke, University of Virginia

28- 10:06  The Deubiquitylase MATH-33 Controls DAF-16 Stability and Function in Development and Longevity. Thomas Heimbucher, Zheng Liu, Carine Bossard, Richard McCloskey, Andrea C. Carrano, Christian G. Riedel, Bogdan Tanasa, Christian Klammt, Bryan R. Fonslow, Celine E. Riera, Bjorn F. Lillemeier, Kenneth Kemphues, John R. Yates III, Clodagh O'Shea, Tony Hunter, Andrew Dillin.

29- 10:18  Insulin-like peptides in sensory neurons transmit inter-tissue longevity signals through modulating DAF-16 activity in C. elegans. M. Artan, D. Jeong, D. Lee, Y. Kim, H. G. Son, J. Alcedo, S.-J. V. Lee.

30- 10:30  CREB-Dependent FLP-6 Neuropeptide Signaling Regulates Longevity Response to Temperature in C. elegans . Yen-Chih Chen, Hung-Jhen Chen, Wei-Chin Tseng, Jiun-Min Hsu, Chun-Liang Pan.

31- 10:42  Environmental temperature differentially modulates C. elegans longevity through a thermosensitive channel. Bi Zhang, Rui Xiao, Elizabeth A. Ronan, Yongqun He, Jianfeng Liu, X. Z. Shawn Xu.

32- 10:54  CYP-36A1 Acts Downstream of the Conserved EGL-9/HIF-1 Hypoxia-response Pathway to Regulate C. elegans Egg-Laying Behavior. Cory Pender, Bob Horvitz.

33- 11:06  Non-autonomous DAF-16/FOXO activity antagonizes age-related loss of C. elegans germline stem/progenitor cells. Zhao Qin, E. Jane Albert Hubbard.

34- 11:18  Investigation of aging and hermaphrodite attractiveness in C. elegans with RNAseq. Daniel Leighton, Paul Sternberg.


Thursday, June 25   8:30 AM–11:30 AM
De Neve Auditorium

Behavior


Chair: Alon Zaslaver, Hebrew University of Jerusalem

35- 8:30  Glia control locomotion and sleep in C. elegans. Menachem Katz, Francis Corson, Shachar Iwanir, Elena Dragomir, David Biron, Shai Shaham.

36- 8:42  NCA sodium leak channels and gap junctions regulate sleep and arousal in C. elegans. Huiyan Huang, Heather Bennett, Chen-Tseh Zhu, Dustin Hayden, Lukas Skuja, Anne Hart.

37- 8:54  Neuropeptide Modulation of Specific Behaviors During EGF/ALA Induced Sleep. Ravi D. Nath, Elly S. Chow, Han Wang, Erich M. Schwarz, Paul W. Sternberg.

38- 9:06  Mechanisms for sleep neuron specification and sleep induction. J. Besseling, M. Turek, J. Spies, H. Bringmann.

39- 9:18  AFD-specific receptor guanylyl cyclases can confer temperature responses onto diverse cell types. Asuka Takeishi*, Yanxun V. Yu*, Vera M. Hapiak, Harold W. Bell, Piali Sengupta.

40- 9:30  Pan-neuronal imaging in roaming animals. Vivek Venkatachalam, Ni Ji, Xian Wang, James Mitchell, Mason Klein, Christopher Tabone, Christopher Clark, Joel Greenwood, Andrew Chisholm, Jagan Srinivasan, Mark Alkema, Mei Zhen, Aravinthan Samuel.

9:42 - Break

Chair: Arantza Barrios, University College London

41- 10:06  Caenorhabditis elegans exhibit a fecal avoidance-like coupling between two motor programs. S. Nagy, Y.-C. Huang, M. J. Alkema, D. Biron.

42- 10:18  The taste receptor homolog LITE-1 is a novel type of photoreceptor protein. Jianke Gong, Yiyuan Yuan, Alex Ward, Zhaoyang Feng, Jianfeng Liu, X. Z.Shawn Xu.

43- 10:30  Dissecting the roles of primary interneurons that regulate memory-dependent salt concentration chemotaxis. Hirofumi Kunitomo, Hirofumi Sato, Yohsuke Satoh, Yuichi Iino.

44- 10:42  Geographical tuning in magnetotactic response across C. elegans wild-type isolates. Andres Vidal-Gadea, Kristi Ward, Celia Beron, Jonathan Pierce-Shimomura.

45- 10:54  The ciliary protein, EFHC1, implicated in epilepsy, functions at the cilium and synapse to modulate dopamine signalling. C. M. Loucks, A. H. McEwan, T. A. Timbers, C. M. Li, D. S. Walker, J. L. Johnson, W. R. Schafer, C. H. Rankin, M. R. Leroux.

46- 11:06  Mechanosensory stimulation controls behaviour and PVD dendritic tree menorah structure. Sharon Inberg, Benjamin Podbilewicz.

47- 11:18  The genetic sex of the chemosensory sensory neuron ADF determines the behavioral response to sex pheromones. Kelli A. Fagan, Jessica R. Bennett, Frank Schroeder, Douglas S. Portman.


Thursday, June 25   8:30 AM–11:30 AM
Northwest Auditorium

Cell Division and Cell Death


Chair: Yonatan Tzur, Hebrew University of Jerusalem

48- 8:30  Compartment-specific killing and clearance programs in the C. elegans tail-spike cell. Piya Ghose, Peter Insley, Yun Lu, Meera Trivedi, Shai Shaham.

49- 8:42  Assisted Suicide: a Caspase- and Engulfment-Dependent Cell Death. Holly Johnsen, Bob Horvitz.

50- 8:54  The Putative TRP Channel CED-11 Functions to Increase Nuclear Membrane Permeability in C. elegans Apoptosis. Kaitlin Driscoll, Gillian Stanfield, Rita Droste, Bob Horvitz.

51- 9:06  Combinatorial control of apoptosis by microRNAs in the C. elegans germline. Anh Tran, Bin Yu, Mehran Haeri, W. Brent Derry.

52- 9:18  Telomere maintenance through recruitment of internal genomic regions. Beomseok Seo, Chuna Kim, Mark Hills, Sanghyun Sung, Hyesook Kim, Eunkyeong Kim, Daisy S. Lim, Hyun-Seok Oh, Rachael Mi Jung Choi, Jongsik Chun, Jaegal Shim, Junho Lee.

53- 9:30  Restriction of Topoisomerase II levels by Aminopeptidase P prevents genome instability. N. Silva, K. Matsuzaki, C. Barroso, D. Brooks, E. R. Isaac, S. J. Boulton, E. Martinez-Perez.

9:42 - Break

Chair: Diana Libuda, University of Oregon

54- 10:06  Karyotype manipulation reveals multiple inputs driving pairwise chromosome synapsis during C. elegans meiosis. B. Roelens, M. Schvarzstein, A. Villeneuve.

55- 10:18  Dynamic phosphoregulation of axis proteins underlies chromosome remodeling during meiosis. Yumi Kim, Scott C. Rosenberg, Nora Kostow, Ofer Rog, Kevin D. Corbett, Abby F. Dernburg.

56- 10:30  Spindle assembly checkpoint proteins regulate and monitor meiotic synapsis in C. elegans. Tisha Bohr, Christian Nelson, Needhi Bhalla.

57- 10:42  Sex-specific features of kinetochore function during sperm meiosis. Vanessa Cota, Luis Quintanilla, Thais Cintra, Byrd Dana, Diana Chu.

58- 10:54  The molecular identification of a gene which controls the distribution of meiotic recombination events in Caenorhabditis elegans. George Chung, Ann Rose, Mark Petalcorin, Nigel O'Neil, Jeffrey Chu, Julie Martin, Zebulin Kessler, Luis Sanchez-Pulido, Chris Ponting, Judith Yanowitz, Simon Boulton.

59- 11:06  C. elegans Chibby-like protein is a SPD-2 interacting centriolar component required for proper SPD-2 localization and centriole duplication. Kenji Sugioka, Danielle R. Hamill, Joshua B. Lowry, Marie E. McNeely, Molly Enrick, Bhavna Murali, Lauren W. Parsons, Bruce Bowerman.

60- 11:18  Regulation of the microtubule severing complex in early C. elegans development. Ryan Smit, Sarah Beard, Benjamin Chan, Paul Mains.


Thursday, June 25   1:00 PM–2:30 PM

Spatial and temporal analysis of gene function in adult C. elegans

Room:Carnesale Palisades Ballroom
Organizers:Coleen Murphy, Princeton University, and
Malene Hansen, Sanford-Burnham Institute

This workshop aims to discuss available techniques for analyzing gene function in select tissues of adult C. elegans. Establishing such approaches is a critical objective for the field to fully understand how specific tissues contribute to organismal phenotypes.

1:00 pm -1:05 pm     Introduction, Coleen Murphy and Malene Hansen
1:05 pm - 1:25 pm     Xingyi She, SBMRI, Protein overexpression in specific tissues
1:25 pm - 1:45 pm     Aaron Reinke, UCSD, Protein localization in specific tissues
1:45 pm – 2:05 pm     David Miller, Vanderbilt, RNA sequencing in specific tissues
2:05 pm – 2:25 pm     Rachel Kaletsky, Princeton, Tissue isolation & transcriptional profiling of specific adult tissues and cells



Thursday, June 25   1:00 PM–2:30 PM

Hours and days: Long-term Imaging of Behavior

Room:Grand Horizon Ballroom
Organizers:Chris Fang-Yen, University of Pennsylvania, and
Anne Hart, Brown University, and
David Raizen, University of Pennsylvania

Optical imaging, microfluidic approaches and other new techniques have expanded the possibilities for using C. elegans to study aging, sleep and other behaviors. In this workshop, eight speakers will present short descriptions of different technical approaches for long term imaging of C. elegans. Speakers will provide specific technical details and discuss costs, expertise required, and scientific/practical considerations for data collection & interpretation. After the short workshop talks are completed, there will be a roughly 30 minute question and discussion period for the audience with all participants.

Schedule of events:
1:00 pm - 1:09 pm      Challenges in tracking single worms for hours to days, David Raizen, University of Pennsylvania
1:10 pm - 1:18 pm      Comparing multi-worm trackers and artificial dirt PDMS chambers for sleep studies, Huiyan Huang, Brown University
1:19 pm - 1:27 pm      Imaging development and aging using the WorMote, Christopher Fang-Yen, University of Pennsylvania
1:28 pm - 1:36 pm      Agarose microchambers for long-term calcium imagin, Henrik Bringmann, Max Planck Institute for Biophysical Chemistry, Göttingen
1:37 pm - 1:45 pm      Microfluidic chamber array for long-term observation of behavior, Yongmin Cho, Georgia Institute of Technology
1:46 pm - 1:54 pm      The Lifespan Machine: learning from 500 terabytes of lifelong video, Nicholas Stroustrup, Harvard Medical School
1:55 pm - 2:03 pm      Imaging individuals from hatching to death in a simple solid-culture system, Zach Pincus, Washington University
2:04 pm - 2:30 pm      Questions/comments from audience and general discussion



Thursday, June 25   1:00 PM–2:30 PM

Advances in quantitative genetics and causal variant identification in C. elegans and other nematodes

Room:De Neve Auditorium
Organizers:Jan Kammenga, Wageningen University, The Netherlands, and
Mark Sterken, Wageningen University, The Netherlands, and
Basten Snoek, Wageningen University, The Netherlands

This workshop aims to bring together laboratories working on or interested in the field of quantitative genetics and natural variation in C. elegans and other nematodes. The quantitative genetics community is rapidly developing new resources and techniques that facilitate the molecular identification of quantitative trait loci. Examples are the use of genome wide association studies together with classical recombinant inbred line screens and the development of high-density introgression strains that facilitate dissection of QTL regions. Furthermore, identification of the underlying quantitative trait nucleotides/genes is also rapidly advancing. The ever increasing availability of mutant strains allows for rapid complementation studies and the development of the CRISPR/CAS9 system makes it possible to induce specific mutations in wild strains. Bringing together groups that are on the forefront of these developments allows for exchange and new combinations of ideas and experiences with all these techniques.

1:00 pm – 1:05 pm      Introduction
1:05 pm – 1:20 pm      Michael O'Donnell, Brandeis University, Natural variation in a TOR-complex 2 component underlies a temperature-dependent polyphenic trait
1:20 pm – 1:35 pm      Erik Andersen, Northwestern University, Strategies to go from QTL to QTG in C. elegans
1:35 pm – 1:50 pm      Daehan Lee, Seoul National University, Natural variations in nictation and identification of responsible QTL
1:50 pm – 2:05 pm      Asher Cutter, University of Toronto, Natural variation and the genetics of adaptive divergence in C. briggsae
2:05 pm – 2:30 pm      Discussion



Thursday, June 25   1:00 PM–2:30 PM

WormBase: Website Usage, Data Mining, and Community Annotation

Room:Northwest Auditorium
Organizer:Chris Grove, CalTech

Schedule:

== Tools and Resources ==

1:00 pm-1:12 pm     Chris Grove: Mining WormBase data with InterMine/WormMine
1:12 pm -1:24 pm     Scott Cain: JBrowse, a new tool for genome browsing in WormBase
1:24 pm -1:36 pm     Raymond Lee: Browsing WormBase ontologies with the new WormBase ontology browser
1:36 pm -1:48 pm     Kevin Howe: ParaSite, Ensembl Genomes, and the UCSC Assembly Hub for C. elegans
1:48 pm -2:00 pm     Kimberly van Auken: Gene Ontology (GO): Finding GO annotations and performing enrichment analysis

== Community Annotation ==

2:00 pm -2:15 pm     Mary Ann Tuli: Contributing variation data to WormBase
2:15 pm -2:30 pm     Ranjana Kishore: Participate in writing gene descriptions for WormBase

For the 2015 International C. elegans meeting, WormBase will present two identical workshops (Thursday and Saturday) to cover some of WormBase's newer tools and data as well as ways in which the nematode research community may contribute data and annotations to the database. We will cover basics of data mining with WormMine (the WormBase instance of Intermine), introduce our instantiation of the JBrowse genome browser, and demo the new ParaSite website which hosts genome sequences for parasitic nematode species. We will discuss WormBase sequence data available in complementary resources such as Ensembl Genomes and the UCSC Assembly Hub for C. elegans and cover the basics of finding Gene Ontology (GO) data in WormBase and performing GO enrichment analysis. We will also provide a number of options for users to submit their own data using sequence variation data and gene concise descriptions as examples.



Thursday, June 25   1:00 PM–2:30 PM

TEM Methods for the Nematode Research

Room:Bradley International Ballroom
Organizers:David Hall, AECOM, and
Irina Kolotuev, Univ. of Rennes, France

Recent advances in technology, preparative methods and analysis are opening new vistas in the anatomy of C. elegans. This workshop will introduce a variety of special techniques that greatly improve our understanding of structure vs function in many nematode tissues.

1:00 pm – 1:12 pm     David Hall, Einstein University, A historical look at previous EM methods and platforms for data sharing, including WormAtlas and WormImage
1:13 pm - 1:25 pm     Yun Lu, Rockefeller University, Improved methods for sample prep, HPF/FS
1:26 pm - 1:38pm     Cristina Berciu and Piali Sengupta, Brandeis University, Electron tomography and serial reconstruction
1:39 pm – 1:51pm     Eddie Hujber, University of Utah, Superresolution and Correlative Light and Electron Microscopy
1:52 pm – 2:04 pm     Irina Kolotuev, University of Rennes, France, Cryo-sectioning and array tomography for immuno-EM labeling
2:05 pm – 2:30 pm     General discussion



Thursday, June 25   3:00 PM–6:00 PM
Royce Hall

Plenary Session 2


Chair: David Fitch, New York University

61- 3:00  Wild worms - a world beyond N2 in its Petri dish. Marie-Anne Felix, Ecole Normale Superieure, CNRS, Inserm, Paris, France.

62- 3:30  X-chromosome evolution: divergence of X-sequence motifs that drive dosage compensation across Caenorhabditis species. Caitlin M. Schartner, Te-Wen Lo, Barbara J. Meyer.

63- 3:42  The molecular signature of animal embryogenesis. Itai Yanai.

64- 3:54  Self-Recognition Prevents Cannibalism in Predatory Nematodes. James Lightfoot, Martin Wilecki, Eduardo Moreno, Vladislav Susoy, Christian Rödelsperger, Ralf Sommer.

65- 4:06  Another update on Caenorhabditis diversity, phylogeny and evolution. Karin Kiontke, Marie-Anne Félix, David H. A. Fitch.

66- 4:12  Long-time quantitative time-lapse microscopy of C. elegans post-embryonic development. Nicola Gritti, Jeroen van Zon.

67- 4:18  The rich get richer: comprehensive quantitative analysis of nuclear SYS-1/β-catenin and POP-1/TCF in C. elegans embryos identifies a novel memory mechanism for gene expression diversification. Amanda L. Zacharias, Travis Walton, Elicia Preston, John Isaac Murray.

4:30 - Break

Chair: Monica Colaiacovo, Harvard Medical School

68- 5:00  Transmitting an epigenetic 'memory of germline' across generations and through cell divisions in C. elegans. Jeremy Kreher, Teruaki Takasaki, Susan Strome.

69- 5:12  A regulatory module involving a microRNA and an RNA binding protein controls sex determination and dosage compensation in the C. elegans embryo. Katherine McJunkin, Victor Ambros.

70- 5:24  De novo lysosome acidification defines a quality control switch in the C. elegans germline. K. Adam Bohnert, Cynthia Kenyon.

71- 5:36  Male Chemosensory Pathways that Modulate Sperm Motility Properties. H. Hoang, M. Miller.

72- 5:48  The P-granule assembly protein, PGL-1, is a base-specific RNA nuclease. Scott Takeo Aoki, Aaron M. Kershner, Marvin Wickens, Craig Bingman, Judith Kimble.


Thursday, June 25   7:30 PM–8:30 PM

Teaching Workshop - What is a PUI and how do I get a job at one?

Room:Northwest Auditorium
Organizer:Jon Karpel, Southern Utah University

Question and answer session with faculty panelists from varied perspectives. Discussion will center around how a PUI (primarily undergraduate institution) differs from other schools, what's it like looking for a job at a PUI now, and how search committees and departments look at candidates.

Panel Participants:
Paula Checchi, Marist College
Jim Lissemore, John Carroll University
Te-Wen Lo, Ithaca College
Jay Pieczynski, Rollins College
Tim Walston, Truman State University



Friday, June 26   8:30 AM–11:30 AM
Carnesale Palisades Ballroom

Physiology: Aging and Stress II


Chair: Stefan Taubert, University of British Columbia

73- 8:30  SKN-1 is essential for evolutionary success during starvation. Dana Lynn, Sean Curran.

74- 8:42  NCL-1; an important player in Dietary Restriction mediated longevity in C. elegans. Varnesh Tiku, Yidong Shen, Bree N. Heestand, Adam Antebi.

75- 8:54  SILeNCe is golden: slow-down of protein turnover in the long-lived Caenorhabditis elegans daf-2 mutant. Ineke Dhondt, Vladislav A. Petyuk, Richard D. Smith, Geert Depuydt, Bart P. Braeckman.

76- 9:06  mRNA decay interfaces with protein synthesis to modulate stress resistance and longevity in C. elegans. Matthias Rieckher, Maria Markaki, Andrea Princz, Nektarios Tavernarakis.

77- 9:18  Loss of Folliculin confers osmotic stress resistance via AMPK-dependent remodeling of carbohydrate stores in C. elegans. Arnim Pause, Elite Possik, Andrew Ajisebutu, Sanaz Manteghi, Mathieu Flamand, Tarika Vijayaraghavan, Barry Coull, Maurice van Stensel, David Hall.

78- 9:30  Neuronal CRTC-1 governs systemic mitochondrial metabolism and lifespan via a catecholamine signal. Kristopher Burkewitz, Ianessa Morantte, Heather Weir, Robin Yeo, Yue Zhang, Frank Huynh, Olga Ilkayeva, Matthew Hirschey, Ana Grant, William Mair.

9:42 - Break

Chair: Jeremy Van Raamsdonk, Van Arpel Institute

79- 10:06  How sirtuins regulate lifespan: endogenous small molecules trigger ROS signaling. Frank C. Schroeder, Andreas Ludewig, Yegeniy Izrayelit, Sydney Campbell, Anne Brunet, Lauren Booth.

80- 10:18  The intrinsic apoptosis pathway mediates the pro-longevity response to mitochondrial reactive oxygen species. Callista Yee, Wen Yang, Siegfried Hekimi.

81- 10:30  Coordination of mitophagy and mitochondrial biogenesis during ageing in C. elegans. Konstantinos Palikaras, Nektarios Tavernarakis.

82- 10:42  Localized redox regulation of IRE-1 kinase activity controls distinct ER and oxidative stress responses. John Hourihan, Lorenza Moronetti Mazzeo, T. Keith Blackwell.

83- 10:54  A novel role for the nuclear receptor NHR-49 in the oxidative stress response. Grace Y. S. Goh, Regina C. Lai, Ka-Young Lee, Stefan Taubert.

84- 11:06  Somatic expression of a germline program does not extend lifespan in C. elegans. Andrew Knutson, Susan Strome.

85- 11:18  NemaFlex: A microfluidic tool for phenotyping (neuro)muscular strength in C. elegans. Mizanur Rahman, Jennifer E. Hewitt, Frank Van Bussel, Jerzy Blawzdziewicz, Nathaniel Szewczyk, Monica Driscoll, Siva A. Vanapalli.


Friday, June 26   8:30 AM–11:30 AM
Grand Horizon Ballroom

Neuronal Development


Chair: Luisa Cochella, Institute of Molecular Pathology

86- 8:30  Tuning of the RNA polymerase II CTD phosphatase SSUP-72 at internal poly(A) sites controls alternative polyadenylation in C. elegans neurons. Fei Chen, Yu Zhou, Yingchuan B. Qi, Vishal Khivansara, Hairi Li, Sang Young Chun, John K. Kim, Xiang-Dong Fu, Yishi Jin.

87- 8:42  Genome-wide analyses of actively translating mRNAs in neurons identify a heat-sensitive transcription elongation factor in a pair of serotonergic sensory neurons. Xicotencatl Gracida, Mike Dion, John A. Calarco.

88- 8:54  Developmental history regulates olfactory behavior via RNAi pathways. Jennie Sims, Maria C. Ow, Mailyn Nishiguchi, Piali Sengupta, Sarah E. Hall.

89- 9:06  Motor Neuron Identity Diversification via Selective Repression of Terminal Selector Target Genes. SY Kerk, P. Kratsios, M. Hart, R. Mourao, O. Hobert.

90- 9:18  Developmental changes in composition of chemotaxis circuits may underlie behavioral maturation. Laura Hale, Eudoria Lee, Daphne Bazopoulou, Nikos Chronis, Sreekanth Chalasani.

91- 9:30  Glial cells instruct neuronal polarity through gap junctions. L. Meng, A. Wan, Y. Jin, D. Yan.

9:42 - Break

Chair: Kota Mizumoto, University of British Columbia

92- 10:06  LON-2/Glypican is a modulator of UNC-6/netrin-mediated axon guidance. Cassandra Blanchette, Paola Perrat, Andrea Thackeray, Claire Bénard.

93- 10:18  Neurons and glia cooperate in assembly of the embryonic C. elegans nerve ring. Georgia Rapti, Shai Shaham.

94- 10:30  Autophagy is required for Axon Outgrowth and the Specification of Presynaptic Sites during Neurodevelopment. Sarah Hill, Andrea Stavoe, Daniel Colon-Ramos.

95- 10:42  MIG-14/Wntless Regulates Dendrite Self-Avoidance Independently of Wnts. Chien-Po Liao, Chun-Liang Pan.

96- 10:54  Spatial control of neurite branching by Wnt-Frizzled/PCP and endosomal signaling. Chun-Hao Chen, Chun-Liang Pan.

97- 11:06  Transcriptional control of postsynaptic remodeling through regulated expression of an immunoglobulin superfamily protein. Siwei He, Alison Philbrook, Rebecca McWhirter, Christopher Gabel, Daniel Taub, Michael Francis, David Miller.

98- 11:18  Temporal regulation of MT dynamics drives synapse remodeling. Naina Kurup, Dong Yan, Alexandr Goncharov, Yishi Jin.


Friday, June 26   8:30 AM–11:30 AM
De Neve Auditorium

Evolution, Ecology, and Germline Development


Chair: John Wang, Biodiversity Research Center, Academia Sinica, Taiwan

99- 8:30  Evolution and development of Caenorhabditis sperm size: from interspecific to intra-individual levels of variation. Anne Vielle, Nuno Soares, Nicolas Callemeyn, Clotilde Gimond, Jeremy C. Gray, Stefan Zdraljevic, Nausicaa Poullet, Céline Ferrari, Patrick McGrath, Erik C. Andersen, Asher D. Cutter, Christian Braendle.

100- 8:42  Developmental fidelity in males varies widely across C. elegans isotypes. Melissa Alcorn, Davon Callander, Bilge Birsoy, Matthew Cieslak, Joel Rothman.

101- 8:54  Assembly of the Caenorhabditis elegans gut microbiota is a deterministic process shaped by the host. Maureen Berg, Ben Stenuit, Joshua Ho, Andrew Wang, Catelin Parke, Matthew Knight, Lisa Alvarez-Cohen, Michael Shapira.

102- 9:06  The diacetyl receptor ODR-10 mediates a natural odor attraction between C. elegans and lactic acid bacteria grown on citrus fruit. Jae Im Choi, Kyoung-hye Yoon, Saraswathi Kalichamy, Sung-Sik Yoon, Jin Il Lee.

103- 9:18  Caenorhabditis briggsae and its two natural viruses, coevolution in a 'ménage à trois'. Lise Frézal, Gautier Brésard, Marie-Anne Félix.

104- 9:30  Never change a running system? A surprising similarity in nematode embryogenesis. Nadin Memar, Katharina Luthe, Sabrina Schiemann, Hanna Chiesielski, Christian Hennig, Barbara Conradt, Ralf Schnabel.

9:42 - Break

Chair: Mara Schvarzstein, Brooklyn College

105- 10:06  A long non-coding RNA acts in developmental timing by repressing LIN-28. Karin Kiontke, R. Antonio Herrera, Edward Vuong, Douglas S. Portman, David H. A. Fitch.

106- 10:18  Multiple mechanisms of germ granule condensation and distribution are essential for soma-germline distinction in the C. elegans embryo. Agata Rybarska, Christian Eckmann.

107- 10:30  Notch signaling antagonizes PRC2-mediated silencing and promotes reprogramming of C. elegans germ cells. Stefanie Seelk, Balázs Hargitai, Irene Kalchhauser, Martina Hajduskova, Silvia Gutnik, Rafal Ciosk, Baris Tursun.

108- 10:42  The NHL-TRIM Protein LIN-41 is a Major Determinant of the Extended Meiotic Prophase of C. elegans Oocytes. Caroline Spike, Tatsuya Tsukamoto, David Greenstein.

109- 10:54  Localized insulin signaling inhibition couples germline stem cell activity to oocyte needs in aging C. elegans adults. Patrick Narbonne, Paul S. Maddox, Jean-Claude Labbé.

110- 11:06  Exogenous regulation of C. elegans germ stem cell proliferation. Alexandra S. Vagasi, Snehal N. Chaudhari, Madhumati Mukherjee, Gaofeng Bi, Mohammad M. Rahman, Jacob Selhub, Edward T. Kipreos.

111- 11:18  The intrinsically-disordered MEG proteins regulate RNA granule assembly and germ cell fate in embryos. Tu Lu, Helen Schmidt, Geraldine Seydoux.


Friday, June 26   8:30 AM–11:30 AM
Northwest Auditorium

Cytoskeleton and Trafficking


Chair: Joshua Bembenek, University of Tennessee

112- 8:30  Competitive Microdomains Hypothesis; using large endosomes of the coelomocyte to investigate subendosomal microdomains. Anne Norris, Alexandra Murr, Julie Gerdes, Simon Wang, Rouchen Ying, Barth Grant.

113- 8:42  DLK-1/p38 MAP Kinase signaling controls cilium length by regulating RAB-5 mediated endocytosis. Aniek van der Vaart, Suzanne Rademakers, Gert Jansen.

114- 8:54  CCM-3/STRIPAK promotes excretory canal extension through endocytic recycling. Benjamin Lant.

115- 9:06  The Non-Muscle Myosin NMY-1 functions with actin polymerizing UNC-34/Enabled to drive contact-dependent dendritic self-avoidance. Lakshmi Sundararajan, Cody Smith, Matthew Tyska, David Miller.

116- 9:18  CDKL-1, a protein related to the human CDKL5 kinase implicated in Rett syndrome and epilepsy, is a novel transition zone protein that controls cilium formation. Kwangjin Park, Chunmei Li, Michel Leroux.

117- 9:30  Post-translational microtubule glutamylation levels control ciliary motor transport, microtubule structure, and cytoskeletal stability. Robert O'Hagan, Malan Silva, Ken Nguyen, Margaret Morash, Sebastian Bellotti, David Hall, Maureen Barr.

9:42 - Break

Chair: Thomas Boulin, CNRS - Universite de Lyon

118- 10:06  LINKIN, a cell adhesion molecule required during collective migration of the male gonad. Mihoko Kato, Tsui-Fen Chou, Collin Z. Yu, John DeModena, Paul W. Sternberg.

119- 10:18  A new role for the conserved centrosomal protein Girdin in the regulation of primary cilia biogenesis in C. elegans and mammalian cells. Inna Nechipurenko, Anique Olivier-Mason, Julie Kennedy, Oliver Blacque, Piali Sengupta.

120- 10:30  Furrowing as the result of mechanically induced actin alignment. Anne-Cecile Reymann, Fabio Staniscia, Anna Erzberger, Guillaume Salbreux, Stephan Grill.

121- 10:42  A network of conserved formins regulates excretory cell tubulogenesis. Daniel Shaye, Iva Greenwald.

122- 10:54  Shaping neurons by twist-tension coupling in chiral cytoskeleton networks. Michael Krieg, Juan G. Cueva, Kerry Spilker, Kang Shen, Alexander R. Dunn, Miriam B. Goodman.

123- 11:06  Distinct contribution of different tubulin isotypes to microtubule dynamics. Yu Honda, Eisuke Sumiyoshi, Asako Sugimoto.

124- 11:18  The fate of the midbody after cell division. Gholamreza Fazeli, Michaela Trinkwalder, Ann Wehman.


Friday, June 26   1:30 PM–4:30 PM
Royce Hall

Plenary Session 3


Chair: Mario de Bono, MRC-LMB

125- 1:30  How cells change shape. Bob Goldstein, University of North Carolina, Chapel Hill.

126- 2:00  PAR polarity in C. elegans zygotes is established by a mechanochemical feedback-patterning motif. Peter Gross, K. Vijay Kumar, Justin S. Bois, Carsten Hoege, Nathan W. Goehring, Frank Jülicher, Stephan W. Grill.

127- 2:12  Asymmetric ubiquitination of the contractile ring by CUL-3 E3 ubiquitin ligase complex regulates asymmetric cytokinesis in P0 cell. Kenji Sugioka, Bruce Bowerman.

128- 2:24  Crossover position directs accurate chromosome remodeling during late meiotic prophase I in Caenorhabditis elegans . Elisabeth Altendorfer, Monica Colaiacovo.

129- 2:36  Dosage Compensation Complex alters X chromosome organization in C. elegans. Rahul Sharma, Jop Kind, Georgina Gomez-Salivar, Bas van Steensel, Peter Askjaer, Peter Meister.

130- 2:48  Regulation of nuclear organization and long-range gene expression by condensin. Bayly Wheeler, Christian Frřkjćr-Jensen, Erika Anderson, Qian Bian, Erik Jorgensen, Barbara J. Meyer.

3:00 - Break

Chair: Asako Sugimoto, Tohoku University

131- 3:30  Whole-brain imaging at cellular resolution reveals multi-neuronal dynamics under non-stimulus condition. Takayuki Teramoto, Terumasa Tokunaga, Osamu Hirose, Yu Toyoshima, Yuichi Iino, Ryo Yoshida, Takeshi Ishihara.

132- 3:36  The C. elegans Cell-Specific Proteomics Toolkit. Kai P. Yuet, Meenakshi K. Doma, Paul W. Sternberg, David A. Tirrell.

133- 3:42  C. elegans Punctin clusters GABAA receptors via neuroligin binding and UNC-40/DCC recruitment. Haijun Tu#, Berangere Pinan-Lucarre#, Tingting Ji, Maelle Jospin, Jean-Louis Bessereau.

134- 3:54  Global brain dynamics generate the holistic motor command pattern in C. elegans. Saul Kato, Harris Kaplan, Tina Schrödel, Manuel Zimmer.

135- 4:06  Deep-proteome & single-worm proteomics pipelines to uncover molecular changes associated with aging. Vikram Narayan, Dalila Bensaddek, Tony Ly, Ehsan Pourkarimi, Megan Laurance, Mark Larance, Anton Gartner, Cynthia Kenyon, Angus Lamond.

136- 4:18  Neuronal Exophers: a novel mechanism for the removal of neurotoxic cytoplasm components. Ilija Melentijevic, Marton Toth, Meghan Arnold, Ryan Guasp, Girish Harinath, Alex Parker, Christian Neri, Monica Driscoll.


Friday, June 26   4:45 PM–5:45 PM
Royce Hall

Keynote Address
Craig Mello, University of Massachusetts Medical School


Chair: Martha Soto, Rutgers University

137- 4:45  A Worm's Tale: Secrets of Inheritance and Immortality. Craig Mello, University of Massachusetts Medical School.


Friday, June 26   8:00 PM–9:00 PM

Teaching Workshop - Navigating the PUI: Teaching vs Research, Politics and Earning Tenure

Room:Northwest Auditorium
Organizer:Jon Karpel, Southern Utah University

Question and answer session with tenured faculty panelists. Panelists will share their experiences concerning the balancing act of teaching, research, and service. Discussion will include topics such as tenure requirements, collaboration, and getting along with others in your department.

Panel Participants:
Greg Hermann, Lewis & Clark College
Philip Meneely, Haverford College
Mary Montgomery, Macalester College
Jennifer Powell, Gettysburg College



Saturday, June 27   8:30 AM–11:30 AM
Carnesale Palisades Ballroom

RNA Interference, Noncoding RNAs, and Genetic Technologies


Chair: Ayelet Lamm, Technion - IIT

138- 8:30  Small RNAs and RNAi machinery mediate transgenerational dauer formation in response to bacterial pathogens in C. elegans. Lidia Verdugo, Fernanda Palominos, Carolina Sanchez, Yessenia Vasquez, Vinicius Maracaja, Francisco Chavez, Andrea Calixto.

139- 8:42  Toward an understanding of cooperative miRNA-mediated silencing. M. N. Flamand, H. H. Gan, E. Wu, A. Vashisht, G. Jannot, J. Wohlschlegel, M. J. Simard, T. F. Duchaine.

140- 8:54  Staufen negatively modulates microRNA activity in Caenorhabditis elegans. Zhiji Ren, Isana Veksler-Lublinsky, Alejandro Vasquez-Rifo, David Morrissey, Victor Ambros.

141- 9:06  Characterization of the Argonaute protein C04F12.1/VSRA-1 in C. elegans development. Monica Wu, Shikui Tu, Zhiping Weng, Julie Claycomb.

142- 9:18  RNA circles can be used to inhibit and assay microRNA activity. Eric Moss, Abrar Sulaimani, Kevin Kemper.

143- 9:30  The Conserved Histone Chaperone, NAP-1 is Required for Small-RNA Mediated Chromatin Modulation. Michelle Francisco, Tuhin Maity, Christopher Wedeles, Julie Claycomb.

9:42 - Break

Chair: Funda Sar, Koc University

144- 10:06  The CSR-1 RNAi pathway promotes germline transcription and defines the chromatin landscape. G. Cecere, S. Hoersch, S. O'Keeffe, R. Sachidanandam, A. Grishok.

145- 10:18  Homologous pairing and unpaired silencing of chromosomes are regulated by RNAi during meiosis in C. elegans. Hiroaki Tabara, Shohei Mitani, Megumi Mochizuki, Yuji Kohara, Kyosuke Nagata.

146- 10:30  WAGO-4, a tissue specific argonaute, plays a role in germline apoptosis and orchestrates post-transcriptional mRNA regulation. Martin Keller, Deni Subasic, Anneke Brummer, Kapil Singh, Luca Ducoli, Shivendra Kishore, Yibo Wu, Mihaela Zavolan, Ruedi Aebersold, Michael Hengartner.

147- 10:42  MORC-1 regulates endogenous small RNAs, chromatin organization, and germline immortality. Natasha Weiser, Danny Yang, Jayshree Khanikar, Suhua Feng, Raymond Chan, Steven Jacobsen, John Kim.

148- 10:54  Computer-assisted transgenesis of C. elegans for deep phenotyping. Cody Gilleland, Adam Falls, James Noraky, Maxwell Heiman, Mehmet Yanik.

149- 11:06  An auxin-inducible degradation (AID) system for precise temporal and spatial control of protein depletion. L. Zhang, J. Ward, Z. Cheng, A. Dernburg.

150- 11:18  Engineering non-mendelian genetics. Henrik Bringmann, Judith Besseling.


Saturday, June 27   8:30 AM–11:30 AM
Grand Horizon Ballroom

Physiology: Metabolism and Pathogenesis


Chair: Jingru Sun, Washington State University

151- 8:30  Acyl-CoA oxidase complexes control the chemical message produced by C. elegans. Xinxing Zhang, Likui Feng, Satya Chinta, Prashant Singh, Yuting Wang, Joshawna Nunnery, Rebecca Butcher.

152- 8:42  Mechanisms underlying transmission dynamics of mutant mitochondrial genomes. Maulik Patel, Harmit Malik.

153- 8:54  New 13C- and 15N-labeling strategies to monitor individual phospholipid dynamics identify regulators of membrane rejuvenation. B. Dancy, S. Chen, R. Drechsler, P. Gafken, C. Olsen.

154- 9:06  Loss of the C. elegans holocarboxylase synthetase homolog, MEL-3 disrupts anterior-posterior polarity in the embryo and causes larval arrest in a diet dependent manner. Jason Watts, Diance Morton, Kenneth Kemphues, Jennifer Watts.

155- 9:18  Dietary vitamin B12 intake dictates expression of a novel, B12-independent propionate breakdown pathway in C. elegans. Emma Watson, Michael Hoy, Maria Olin-Sandoval, Markus Ralser, A. J. Marian Walhout.

156- 9:30  Innate immunity mediated longevity and reproductive longevity converge on the c-type lectin domain (ctld) protein UPR-1. Elad Yunger, Modi Safra, Yehuda Salzberg, Sivan Henis-Korenblit.

9:42 - Break

Chair: Jonathan Ewbank, CNRS/CIML

157- 10:06  Internal structural damage induces an innate immune response in C. elegans epidermis. Yun Zhang, Wenna Li, Linfeng Li, Yuanbao Li, Rong Fu, Yi Zhu, Huimin Zhang.

158- 10:18  The gut-brain cross talk regulates innate immunity in Caenorhabditis elegans. Abdul Hakkim Rahamathullah, Sakthimala Jagadeesan, Sid Ahmed, Rhonda Feinbaum, Fred Ausubel.

159- 10:30  Aberrant activation of p38 MAP kinase-dependent innate immune responses is toxic to C. elegans. Hilary Cheesman, Rhonda Feinbaum, Robert Dowen, Read Pukkila-Worley.

160- 10:42  Mitophagy and hypoxic response protect C. elegans against P. aeruginosa pathogenesis. Natalia Kirienko, Daniel Kirienko, Fred Ausubel, Gary Ruvkun.

161- 10:54  Opposite immune responses in Caenorhabditis elegans caused by a single gene, the neuropeptide receptor gene npr-1. R. Nakad, B. L. Snoek, W. Yang, K. Dierking, P. C. Rosenstiel, J. E. Kammenga, H. Schulenburg.

162- 11:06  A STAT homologue is a key transcription factor in Caenorhabditis elegans antiviral immunity. Melanie Tanguy, Peter Sarkies, Eric Miska.

163- 11:18  Infection by a single microsporidia cell fuses the intestine of C. elegans into a syncytium. Keir Balla, Emily Troemel.


Saturday, June 27   8:30 AM–11:30 AM
De Neve Auditorium

Regeneration and Synaptic Function


Chair: Michael Ailion, University of Washington

164- 8:30  The microtubule dynamics regulator EFA-6 responds to injury and inhibits axon regeneration via TAC-1 and ZYG-8/DCLK. Lizhen Chen, Marian Chuang, Thijs Koorman, Mike Boxem, Yishi Jin, Andrew Chisholm.

165- 8:42  EFF-1-mediated regenerative axonal fusion requires components of the apoptotic pathway. Brent Neumann, Sean Coakley, Rosina Giordano-Santini, Casey Linton, Eui Seung Lee, Akihisa Nakagawa, Ding Xue, Massimo A. Hilliard.

166- 8:54  Identification of a new regulator of age-dependent axon regeneration by characterizing the neuronal IIS/FOXO transcriptome from isolated adult C. elegans neurons. Vanisha Lakhina, Rachel Kaletsky, April Williams, Rachel Arey, Jessica Landis, Jasmine Ashraf, Coleen Murphy.

167- 9:06  PARGs and PARPs: Novel Regulators of Axon Regeneration. Alexandra B. Byrne, Rebecca D. McWhirter, David M. Miller, III, Marc Hammarlund.

168- 9:18  AMPK modulates the activity of neural circuits to trigger adaptive behavioral outcomes during periods of starvation . Moloud Ahmadi, Richard Roy.

169- 9:30  The Johanson-Blizzard Syndrome Ubiquitin Ligase UBR-1 Regulates Glutamate Metabolism and Signaling. Jyothsna Chitturi, Maria A. Lim*, Wesley L. Hung*, Anas M. Abdel Rahman, John Calarco, Renee Baran, Xun Huang, James Dennis, Mei Zhen.

9:42 - Break

Chair: Brent Neumann, Monash University

170- 10:06  A two-tier glutamate clearance system in C. elegans preserves signaling fidelity and circuit separation in the absence of synaptic isolation. KyungWha Lee, Jenny Chan-Ying Wong, Itzhak Mano.

171- 10:18  Carbon dioxide sensing controls CREB-dependent regulation of neuropeptide expression. Teresa Rojo Romanos, Jakob Gramstrup Petersen, Roger Pocock.

172- 10:30  The C. elegans RID neuron is a neurosecretory cell that modulates locomotion. Wesley Hung*, Maria A. Lim*, Jyothsna Chitturi*, Valeriya Laskova, Yangning Lu, Douglas Holmyard, Rebecca McWhirter, Ni Ji, John Calarco, Chi-Yip Ho, Aravinthan Samuel, David M. Miller, Mei Zhen.

173- 10:42  Stress induced plasticity of the C. elegans electrical synapse network. Abhishek Bhattacharya, Daehan Lee, Junho Lee, Oliver Hobert.

174- 10:54  CLH-1 is a bicarbonate permeable anion channel involved in amphid sheath glia pH regulation. Jeff Grant, Cristina Matthewman, Christina Johnson, Laura Bianchi.

175- 11:06  Nano-electrode recordings in intact C. elegans reveal phenotypes for neurological disease models. Daniel Gonzales, Krishna Badhiwala, Ben Avants, Jacob Robinson.

176- 11:18  Glycolytic enzymes localize to synapses under energy stress to support synaptic function. SoRi Jang, Jessica C. Nelson, Eric G. Bend, Lucelenie Rodríguez-Laureano, Felipe G. Tueros, Luis Cartagenova, Katherine Underwood, Erik M. Jorgensen, Daniel A. Colón-Ramos.


Saturday, June 27   8:30 AM–11:30 AM
Northwest Auditorium

Cell Fate, Differentiation and Morphogenesis


Chair: Tina Gumienny, Texas Woman's University

177- 8:30  Safeguarding cell fates by the FACT histone chaperone network. Ena Kolundzic, Martina Hajduskova, Baris Tursun.

178- 8:42  Dimerization-driven degradation of C. elegans and human E proteins in C. elegans gonadogenesis. Maria Sallee, Iva Greenwald.

179- 8:54  Centrosome-associated degradation limits SYS-1/β-catenin inheritance after asymmetric cell division. Setu Vora, Bryan Phillips.

180- 9:06  Terminal Selector Transcription Factors Orchestrate Cell Fate Restriction in Addition to Specification. Tulsi Patel, Oliver Hobert.

181- 9:18  Hedgehog and PTCHD genes couple reactivation of quiescent neural progenitors to the dietary environment. Masamitsu Fukuyama, Masahiko Kume, Kenji Kontani, Toshiaki Katada.

182- 9:30  The conserved kinases MPK-1, GSK-3, CDK-4 and CDK-2 promote LIN-45/Braf protein turnover in a dynamic spatial and temporal pattern. Claire de la Cova, Iva Greenwald.

9:42 - Break

Chair: Fumio Motegi, Temasek Life Sciences Laboratory

183- 10:06  Making the gonad to be mirror-symmetric by Wnt signaling. Shuhei So, Hitoshi Sawa.

184- 10:18  LET-502/ ROCK acting independently of myosin-II promotes junctional protein transport or trafficking in parallel to microtubules. Sophie Quintin, Christelle Gally, Shaohe Wang, Karen Oegema, Michel Labouesse.

185- 10:30  EGF/Ras signaling and the AFF-1 fusogen control seamless tube auto-fusion and shaping in the C. elegans excretory system. F. Soulavie, M. Sundaram.

186- 10:42  FBN-1, a fibrillin-related protein, promotes resistance of the epidermis to mechanical deformation during embryogenesis. Melissa Kelley, John Yochem, Michael Krieg, Andrea Calixto, Maxwell Heiman, Aleksandra Kuzmanov, Vijaykumar Meli, Martin Chalfie, Miriam Goodman, Shai Shaham, Alison Frand, David Fay.

187- 10:54  A Rho-specific GAP functions in response to axonal guidance signals to modulate the actin cytoskeleton during embryonic morphogenesis. Andre Wallace, Martha Soto.

188- 11:06  A conserved RNA binding protein collaborates with specific miRNAs to regulate C. elegans development. Rebecca Zabinsky, Brett Weum, Ben Weaver, Yi Weaver, Min Han.

189- 11:18  Regulation of embryonic genome activation by endoRNAi. Christina Fassnacht, Cristina Tocchini, Jorge Merlet, Michael Stadler, Rafal Ciosk.


Saturday, June 27   1:00 PM–2:30 PM

High-throughput Sequencing Based Techniques

Room:Carnesale Palisades Ballroom
Organizer:Sevinc Ercan, New York University

This workshop is designed to help researchers who wish to use high-throughput genomics to study gene regulation and want to find out where to start. We also encourage participation from experienced researchers who would like to contribute to the discussion. We will cover ChIP-seq, RNA-seq, and small RNA-seq techniques. For each, we will discuss experimental issues such as sample preparation and controls; analysis issues such as normalization; data interpretation and discussion of the strength and weaknesses of each technique.

Schedule of events:

1:00 pm -1:05 pm      Introduction, Sevinc Ercan, NYU
1:05 pm -1:30 pm      ChIP-seq, Sevinc Ercan, NYU
1:30 pm -2:00 pm      RNA-seq, Erin Osborne, UNC
2:00 pm -2:30 pm      small RNA-seq, Weifeng Gu, UC Riverside



Saturday, June 27   1:00 PM–2:30 PM

Worm Tracking: Recent Advances and Future Directions

Room:Grand Horizon Ballroom
Organizer:Andre Brown, Imperial College London

Published tracking methods have been designed for different, but often overlapping, purposes and they each have advantages and disadvantages, including differing degrees of availability and adaptability. Driven by the desire for real-time analysis, higher-throughput, and more informative features, there are still a number of labs working on developing the next generation of worm trackers. This workshop will have three purposes:

1) To share recent advances in worm tracking with the broader community
2) To get feedback from the community on what new technologies would be most useful in their work
3) To discuss the possibility of coordinating efforts where possible and to consider working towards a more universal open source worm tracker that can serve as a shared basis for future developments

Schedule

1:00 pm – 1:12 pm      Motorized-stage tracking, Weiwei Zhong, Rice University
1:12 pm – 1:24 pm     High-throughput multi-worm tracking, Rex Kerr, UCSF
1:24 pm – 1:36 pm     Locomotion and posture on developmental timescales, David Biron, University of Chicago
1:36 pm – 1:48pm     Quantifying swimming, Monica Driscoll, Rutgers University
1:48 pm – 2:00pm     Tracking turns and tangles in the crossed body movements of C. elegans, Greg Stephens, VU Amsterdam
2:00 pm – 2:30pm      Discussion



Saturday, June 27   1:00 PM–2:30 PM

Integrating Methods in C. elegans Structural Neurobiology and Cell Anatomy from Embryos to Adults

Room:De Neve Auditorium
Organizers:Andrew Chisholm, University of California, San Diego, and
Aravi Samuel, Harvard University, and
Mei Zhen, Samuel Lunenfeld Research Institute

The anatomical foundation of much C. elegans research derives from classic light and electron microscopy studies in the 1970s and 1980s. More modern methods of EM reconstruction are now extending these studies to different stages and conditions. The first half of this workshop will present and discuss methods and challenges in large-scale and high-throughput EM anatomy.

The stereotyped anatomy of C. elegans allows unique identification of cells. However without extensive training such identification remains challenging. The second half of the workshop will focus on progress in manual and automation of cell ID, mostly in light/fluorescence microscopy datasets, in embryos and larvae/adults. Cell ID in the nervous system and other tissues will be discussed. We encourage participants to bring their own cell ID challenges, questions, and solutions to the workshop.

1:00 pm -1:05 pm Introduction (A Chisholm)

EM session 1:05 pm -1:30pm
1) EM dataset processing and alignment: (Ben Mulcahy, Zhen lab)
2) EM Databases and Data Analyses (Steven Cook, Emmons/Hall lab)
3) Approaches to EM segmentation (Daniel Witvliet, Zhen lab; James Mitchell, Samuel lab)

Open discussion (1:30 pm-1:45pm)

Microscopy session 1:45 pm-2:10 pm
1) Cell tracking and ID in embryos (Bao lab)
2) Manual neuronal ID in larvae and adults (Hobert lab)
3) Automated tracking and ID in behaving animals (Hobert; Zimmer; Samuel labs)

Open discussion (2:10 pm -2:25 pm)

2:25 pm -2:30 pm Closing Remarks (Mei Zhen)



Saturday, June 27   1:00 PM–2:30 PM

WormBase: Website Usage, Data Mining, and Community Annotation

Room:Northwest Auditorium
Organizer:Chris Grove, CalTech

Schedule:

== Tools and Resources ==

1:00 pm-1:12 pm     Chris Grove: Mining WormBase data with InterMine/WormMine
1:12 pm -1:24 pm     Scott Cain: JBrowse, a new tool for genome browsing in WormBase
1:24 pm -1:36 pm     Raymond Lee: Browsing WormBase ontologies with the new WormBase ontology browser
1:36 pm -1:48 pm     Kevin Howe: ParaSite, Ensembl Genomes, and the UCSC Assembly Hub for C. elegans
1:48 pm -2:00 pm     Kimberly van Auken: Gene Ontology (GO): Finding GO annotations and performing enrichment analysis

== Community Annotation ==

2:00 pm -2:15 pm     Mary Ann Tuli: Contributing variation data to WormBase
2:15 pm -2:30 pm     Ranjana Kishore: Participate in writing gene descriptions for WormBase

For the 2015 International C. elegans meeting, WormBase will present two identical workshops (Thursday and Saturday) to cover some of WormBase's newer tools and data as well as ways in which the nematode research community may contribute data and annotations to the database. We will cover basics of data mining with WormMine (the WormBase instance of Intermine), introduce our instantiation of the JBrowse genome browser, and demo the new ParaSite website which hosts genome sequences for parasitic nematode species. We will discuss WormBase sequence data available in complementary resources such as Ensembl Genomes and the UCSC Assembly Hub for C. elegans and cover the basics of finding Gene Ontology (GO) data in WormBase and performing GO enrichment analysis. We will also provide a number of options for users to submit their own data using sequence variation data and gene concise descriptions as examples.



Saturday, June 27   1:00 PM–2:30 PM

Caenorhabditis Genomes Project

Room:Bradley International Ballroom
Organizer:Mark Blaxter, University of Edinburgh

The sequencing of the genome of the nematode C. elegans remains one of the milestones of modern biology, and this genome sequence is the essential backdrop to a vast body of work on this key model organism. As Dobzhansky said, “Nothing in biology makes sense except in the light of evolution”, and it is clear that complete understanding of C. elegans will only be achieved when it is placed in an evolutionary context. The field of comparative nematode genomics is now moving forward rapidly, with many new species of Caenorhabditis having been identified in the last decade, and new initiatives to explore the genomics and population genomics of the genus. In particular an open "Caenorhabditis Genomes Project" has been initiated to generate draft genome sequence for the majority of the remaining unsequenced species.

This workshop will

- provide context and overview of the need for and promise of a Caenorhabditis genomes project
- review the current state of the art in Caenorhabditis species discovery, diversity and genomics
- bring participants fully up-to-date with progress and plans
- present vignettes of current research being done with the new diversity of genomes
- encourage and consolidate community opinion, needs and plans

Schedule:

1:00 pm      Welcome, Mark Blaxter, organiser
1:05 pm      The phylogeny of Caenorhabditis, Karin Kiontke, NYU
1:20 pm      What will a fully sequenced phylogeny of Caenorhabditis do for me?, TBC
1:40 pm      Current status of the CGP, Georgios Koutsovoulos, University of Edinburgh
1:50 pm      Improving Caenorhabditis genome assembly with long read data, Erich Schwarz, Cornell University
2:00 pm      Community guided discussion: What do we need from the Caenorhabditis Genomes Project?
2:20 pm      Community taskforce: Who will do what to make this vision a reality?



Saturday, June 27   1:30 PM–3:00 PM
West Coast

Plenary Session and Workshop for Undergraduate Researchers


Chair: Beth Ruedi, GSA

Undergraduate conference attendees will attend a plenary session with two talks presented at a level appropriate for an undergraduate audience. Participants will then have a chance to talk to a panel of graduate students about applications, interviewing, admission, choosing a lab and quality of life in graduate school.





Saturday, June 27   2:30 PM–4:30 PM

Teaching Workshop: Teaching Resources Available to Incorporate C. elegans into the Classroom

Room:Northwest Auditorium
Organizer:Jon Karpel, Southern Utah University

Workshop attendees will be introduced to several different resources available to teachers on how to use this model organism in the classroom. Louisa Stark, recipient of the 2015 Elizabeth W. Jones Award for Excellence in Education, will present the Learn.Genetics and Teach.Genetics resources.

Panel Participants:
Louisa Stark, University of Utah
Elizabeth Ruedi, Education Director, GSA
Elizabeth Ann De Stasio, Senior Editor of Genetics, Lawrence University



Sunday, June 28   9:00 AM–10:15 AM
Royce Hall

Plenary Session 4


Chair: Sophie Jarriault, IGBMC

190- 9:00  Vulval development: a model for tube morphogenesis. Alex Hajnal, University of Zurich, Switzerland.

191- 9:30  Epidermal wounding induces mitochondrial ROS production that promotes wound repair in C. elegans. Suhong Xu, Andrew D. Chisholm.

192- 9:42  Regulation of mitochondrial fusion in physical exertion and lifespan extension. Snehal N. Chaudhari, Edward T. Kipreos.

193- 9:54  Noxious Stimuli Induce Spitting by C. elegans via Spatially-Restricted Calcium Increases in Pharyngeal Muscle. Steve Sando, Nikhil Bhatla, Bob Horvitz.

10:06 - Break




Sunday, June 28   10:30 AM–12:00 NOON

CRISPR-based Strategies for Genome Engineering - the "CRISPR Revolution"

Room:Royce Hall
Organizers:Mike Boxem, Utrecht University, and
Daniel Dickinson, University of North Carolina, and
Alexandre Paix, Johns Hopkins University School of Medicine

In just a few years' time, CRISPR-based genome engineering has become an essential tool for many C. elegans groups. This exciting technology is still rapidly evolving, with new insights being gained on an almost daily basis. This workshop offers an opportunity to learn about the latest developments in CRISPR/Cas9 genome engineering, share ideas, and gain practical tips, protocols, and insights to enable the successful application of this technology. In a series of short talks, researchers actively developing novel methods or improvements will present their work, with a focus on practical, technical advice. Topics that will be discussed include improvements to efficiency and selection of genome edits, and novel Cas9 applications. The talks will be followed by a Q&A session. The workshop will end with a summarizing overview of the field by Geraldine Seydoux.


Speakers:

10:30 am   Introduction. Mike Boxem.

10:35 am   Cloning-free genome editing using Cas9-guide RNA ribonucleoprotein complexes. Alexandre Paix, Andrew Folkmann, Dominique Rasoloson, Jarrett Smith, Geraldine Seydoux.

10:43 am   Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design. Behnom Farboud, Barbara Meyer.

10:51 am   Improvements and challenges with pha-1(ts) co-conversion. Jordan Ward, Liangyu Zhang, Abby Dernburg.

10:59 am   Streamlined CRISPR-based genome engineering with a self-excising drug selection cassette. Daniel J. Dickinson, Ariel M. Pani, Jennifer K. Heppert, Christopher D. Higgins, Bob Goldstein.

11:07 am   Activation of endogenous gene transcription using Cas-9 in C. elegans. Joseph Zullo, Noah Davidsohn, Alejandro Chavez, Monica Colaiacovo, George Church, Bruce Yankner.

11:15 am   CRISPR/Cas9-mediated gene knock-out and knock-in applications in C. elegans. Zhiping Wang, Yishi Jin.

11:23 am   Q&A. with the speakers.

11:50 am   An Overview of CRISPR-based Genome Engineering. Geraldine Seydoux.