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2014 Important Dates

Abstract Submission Opens January 8
Conference Registration Opens January 15
Abstract Submission Deadline (extended) March 17
12 pm ET
Early Conference Registration  & Housing Deadline May 8
Abstract Revision Deadline (extended) March 18
Platform/Poster Assignments Online May 1

Poster Session Listing

 

 

 

POSTER SESSIONS

Mechanism and regulation of flagellar motility

84
FAP174 is an MYCBP-1-like protein that interacts with a central pair A-Kinase Anchoring Protein 240. Jacinta D'Souza1, Venkatramanan Rao1, Ruhi Sarafdar1, Twinkle Choudhary1, Priyanka Sivadas2, Pinfen Yang2. 1) UM-DAE Ctr for Excellence in Basic Sciences, Mumbai, Maharashtra, India; 2) Marquette University, Wehr Life Sciences, P.O. Box 1881, Milwaukee, WI 53201-1881, (414) 288-7355.


Assembly of the flagellar axoneme

85
Life at the tip: IFT-independent transport and exchange of the ciliary tip protein EB1-GFP. Aaron J. Harris1, Yi Liu2, Pinfen Yang2, Karl Lechtreck1. 1) Cellular Biology, University of Georgia, Athens, GA; 2) Department of Biological Sciences, Marquette University, Milwaukee, WI.

86
A fap133 mutant gives new insights into dynein 1b. Y. Hou1, S. M. King2, K. F. Lechtreck3, G. B. Witman1. 1) Dept. of Cell and Developmental Biology, Univ. of Massachusetts Medical School, Worcester, MA; 2) Dept. of Molecular, Microbial, and Structural Biology, Univ. of Connecticut Health Center, Farmington, CT; 3) Dept. of Cellular Biology, Univ. of Georgia, Athens, GA.

87
Characterization of an ift46 suppressor mutant shows that the N-terminus of intraflagellar transport protein IFT46 is critical for importing outer arm dynein into flagella. Y. Hou, G. B. Witman. Dept. of Cell and Developmental Biology, Univ. of Massachusetts Medical School, Worcester, MA.

88
The motility mutant pf23 is defective in the DYX1C1 gene and fails to assemble the ciliary inner arm dyneins. Ryosuke Yamamoto1, Stephen M. King2, Winfield S. Sale1, Susan K. Dutcher3. 1) Dept Cell Biol, Emory Univ Sch Medicine, Atlanta, GA; 2) Dept. of Molecular Biology and Biophysics, Univ. of CT Health Science Center, Farmington CT; 3) Dept. of Genetics, Wash. Univ. Sch of Medicine, St. Louis MO.


Flagellar length regulation, basal bodies, and transition zone

89
The uncovering of several new deflagellation genes and the identification of ADF1. Paul Buckoll, Laura Hilton, Fabian Meili, Jaime Kirschner, Julie Rodriguez, Courtney Choutka, Lynne Quarmby. Simon Fraser University, Burnaby, BC, Canada.

90
Quantitative proteomic analysis of differentially expressed proteins in a LF mutant and wild type cells of Chlamydomonas reinhardtii. Dilani Gunasena, Nedra Wilson. Department of Anatomy & Cell Biology, Oklahoma State University Center for Health Sciences, Tulsa, OK 74107.

91
Transcriptome analysis of shf mutants. Aidan Huene1,2, Nedra Wilson2. 1) Dept Biology and Chemistry, Oral Roberts University, Tulsa, OK; 2) Dept Anatomy and Cell Biology, Oklahoma State University Center for Health Sciences, Tulsa, OK.

92
Axonemal tubulin polyglutamylation modulates flagellar length by enhancing tubulin turnover at the flagellar tip. Tomohiro Kubo1,2, Masafumi Hirono2, Takumi Aikawa2, Ritsu Kamiya2,3, George B. Witman1. 1) Dept. Cell and Developmental Biology, Univ. of Massachusetts, Worcester, MA; 2) Dept. Biological Science, Graduate School of Science, Univ. of Tokyo, Tokyo, Japan; 3) Dept. of Life Science, Faculty of Science, Gakushuin Univ., Tokyo, Japan.

93
Identification of the SHF1 gene using a “directed” whole genome sequencing approach. Dylan D. Smith, Nedra Wilson. College of Health Sciences, Oklahoma State University, 1111 W 17th St, Tulsa, OK.

94
Role of GSK3 and LiCl in flagellar regeneration and elongation. L. Wang, J. Pan. School of Life Sciences, Tsinghua University, Beijing, Beijing, China.


Photosynthesis

95
Microalgal photosynthesis coupled to cell motion in a photobioreactor: kinetic model and dynamic simulation. Claude Aflalo. Microalgal Biotechnology Lab, Ben Gurion University, Midreshet Ben Gurion, Negev, Israel.

96
Molecular characterization of lutein-deficient Chlamydomonas mutant with altered non-photochemical quenching. KwangRyul Baek, Minjung Kim, EonSeon Jin. Dept. of Life science, Hanyang University, Seoul, South Korea.

97
Isolation and Characterization of a High Pmax Mutant of Chlamydomonas reinhardtii . Yong Bai1, Graham Peers2, Krishna K. Niyogi1,3. 1) Howard Hughes Medical Institute, Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA; 2) Department of Biology, Colorado State University, Fort Collins, Colorado 80523, USA; 3) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

98
Molecular Characterization of Chlb-lacking mutants in Chlamydomonas reinhardtii. Sandrine Bujaldon1, Natsum Kodama2, Subramanyam Rajagopal3, Yuichiro Takahashi2, Francis-André Wollman1. 1) CNRS/UPMC UMR7141, Institut de Biologie Physico-Chimique, Paris, France; 2) Okayama University, Japan; 3) University of Hyderabad, India.

99
A conserved rubredoxin in the thylakoid membrane is required for photosystem II accumulation. Robert Calderon1, Catherine de Vitry2, José García-Cerdán1, Alizée Malnoë1, Ron Cook1, Rachel Dent1, James Russell1, Cynthia Gaw1, Francis-André Wollman2, Danja Schünemann3, Krishna Niyogi1. 1) Howard Hughes Medical Institute, Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA; 2) Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141, Centre National de la Recherche Scientifique - Université Paris 6, 75005 Paris, France; 3) AG Molekularbiologie Pflanzlicher Organellen, Ruhr-Universität-Bochum, 44801 Bochum, Germany.

100
The fast and slow reversible fluorescence quenching (in vivo) of LHC complexes in Chlamydomonas reinhardtii. Emine Dinc, Lijin Tian, Roberta Croce. Department of Physics and Astronomy, Faculty of Sciences, Vrije University Amsterdam, 1081 HV Amsterdam, The Netherlands.

101
The BSD2 homolog in Chlamydomonas reinhardtii is a polysome-associated chaperone of nascent polypeptides encoding the Rubisco large subunit. L. Doron, N. Segal, M. Shapira. life sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

102
The nucleus encoded TPR protein Mac1 is a novel regulator of PSI gene expression in the chloroplast. Damien Douchi1, Linnka Lefebvre-Legendre1, Xenie Johnson2, Jean-David Rochaix1, Michel Goldschmidt-Clermont1. 1) Department of Botany and Plant Biology, University of Geneva, Switzerland; 2) UMR 7141, CNRS/Université Pierre et Marie Curie, Institut de Biologie Physico-Chimique, Paris, France.

103
Strategies of Chlamydomonas reinhardtii to cope with fluctuating light. Martina Jokel1, Gilles Peltier2,3, Yaghut Allahverdiyeva-Rinne1, Eva Mari Aro1. 1) University of Turku, Department of Biochemistry, Molecular Plant Biology, Biocity 6th floor, Turku 20520, Finland; 2) CEA, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues, CEA Cadarache, Saint-Paul-lez-Durance, F-13108 France; 3) Aix Marseille Université, UMR Biologie Végétale et Microbiologie Environnementale, Saint-Paul-lez-Durance, F-13108 France.

104
Heme ci of the cytochrome b6f complex- an adaptation to oxygenic environment. Alizée Malnoë1,2,3, Jacqueline Girard-Bascou1, Frauke Baymann4, Daniel Picot5, Jean Alric1,6, Daniel Olof Persson7, Francis-André Wollman1, Fabrice Rappaport1, Catherine de Vitry1. 1) CNRS/UPMC UMR7141, IBPC, 13 rue Pierre et Marie Curie 75005 Paris, FRANCE; 2) Howard Hughes Medical Institute, Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA; 3) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; 4) CNRS/AMU UMR7281, BIP, 13402 Marseille, FRANCE; 5) CNRS/UP7 UMR 7099, IBPC, 13 rue Pierre et Marie Curie 75005 Paris, FRANCE; 6) CEA/AMU, IBEB, 13108 Saint-Paul-Lez-Durance, FRANCE; 7) Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Copenhagen, DENMARK.

105
In vitro and in vivo analysis of LHCII proteins in Chlamydomonas reinhardtii. Alberto Natali. VU University, Amsterdam, Netherlands.

106
Self-adjusting light harvesting antenna: New ways to deal with the hazards of harnessing sunlight. Sangeeta Negi1, Zoee Perrine2, Anil Kumar2, Amanda Barry3, Richard Sayre1,3. 1) New Mexico Consortium,Los Alamos,NM; 2) Phycal,Inc St. Louis,MO; 3) Los Alamos National Lab, Los Alamos,NM.

107
Deficiency in cyclic electron flow in a Chlamydomonas pgrl1 knock-out mutant is compensated by combined increases in mitochondrial cooperation and oxygen photoreduction. Kieu-Van Dang1, Julie Plet1, Dimitri Tolleter1, Martina Jokel2, Pierre Richaud1, Yagut Allahverdiyeva2, Xenie Johnson1, Jean Alric1, Gilles Peltier1. 1) CEA, CNRS, Aix-Marseille Université, Institut de Biologie Environnementale et Biotechnologie, CEA Cadarache, Saint-Paul-lez-Durance, F-13108 France; 2) Laboratory of Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014, Turku, Finland.

108
Alterations of photosynthetic apparatus during photoautotrophic growth of Chlamydomonas. M. Sandmann1, O. Reich1, M. Steup2. 1) University of Potsdam, Institute of Chemistry, Potsdam, Germany; 2) University of Potsdam, Institute of Biochemistry and Biology, Potsdam, Germany.

109
Improved photoautotrophic growth of chloroplast transformants in which chloroplast ycf4 gene was overexpressed. S. Nellaepalli1,2, H. Kuroda1,2, Y. Takahashi1,2. 1) The Graduate School of Natural Science and Technology, Okayama Univ, Okayama, Japan; 2) JST-CREST.

110
Regulation of LHCSR3 expression in Chlamydomonas reinhardtii. Ryutaro Tokutsu1, Dimitris Petroutsos2, Shinichiro Maruyama1, Andre Greiner3, Tilman Kottke4, Maria Mittag5, Peter Hegemann3, Giovanni Finazzi2, Jun Minagawa1. 1) Division of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Japan; 2) Cell and Plant Physiology Laboratory, iRTSV, CEA Grenoble, France; 3) Institute for Experimental Biophysics, Humboldt University, Berlin, Germany; 4) Department of Chemistry Physical and Biophysical Chemistry, Bielefeld University, Bielefeld, Germany; 5) Institute of General Botany and Plant Physiology, Friedrich Schiller University, Jena, Germany.

111
Characterization of Rubisco assembly-dependent regulation in Chlamydomonas reinhardtii. Wojciech Wietrzynski1, David B. Stern2, Francis-André Wollman1, Katia Wostrikoff1,2. 1) UMR7141, CNRS-UPMC, IBPC, Paris, France; 2) Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, USA.

112
Characterization of CPLD49, a GreenCut redox protein implicated in cytochrome b6f complex accumulation and the delay of ‘senescence’ in Chlamydomonas reinhardtii. Tyler Wittkopp1,2, Xenie Johnson1, Wenqiang Yang1, Mark Heinnickel1, Krishna Niyogi3, Francis-André Wollman4, Arthur Grossman1,2. 1) Dept of Plant Biology, Carnegie Institution for Science, Stanford, CA; 2) Dept of Biology, Stanford University, Stanford, CA; 3) Dept of Plant and Microbial Biology, University of California, Berkeley, CA; 4) Institut de Biologie Physico-Chimique, Paris, France.

113
Quantitative growth measurement of individual mutants in pooled culture of Chlamydomonas to identify mutants deficient in photosynthesis and photoprotection. Ru Zhang, Nina Ivanova, Leif Pallesen, Luke Mackinder, John Nguyen, Weronika Patena, Xiaobo Li, Rebecca Yue, Spencer Gang, Sean Blum, Arthur Grossman, Martin Jonikas. Plant Biology, Carnegie Institution for Science, Stanford, CA. 94305, USA.


Chloroplast biogenesis and function

114
Molecular insights into the functional role of the rubredoxin domain-containing protein RBD1 in the assembly of Photosystem II. Jose Gines Garcia Cerdan1, Robert H. Calderon1, Danja Schünemann2, Krishna K. Niyogi1,3. 1) UC Berkeley-HHMI, Berkeley, CA; 2) AG Molekularbiologie Pflanzlicher Organellen, Ruhr-Universität-Bochum, 44801 Bochum, Germany; 3) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

115
A factor involved in chloroplast group II intron trans-splicing exhibits intrinsic RNase activity. Jessica Jacobs, Christina Marx, Olga Reifschneider, Stephanie Glanz, Ulrich Kück. General and Molecular Botany, Ruhr Univ Bochum, Bochum, Germany.

116
The structure of chloroplast nucleoids in the green alga Chlamydomonas reinhardtii. Y. Kobayashi1, M. Takusagawa1, M. Odahara2, Y. Fukao3, T. Shikanai1, Y. Nishimura1. 1) Kyoto Univ., Kyoto, Japan; 2) Dep. Life Sci., Coll. Sci., St. Paul's Univ., Tokyo, Japan; 3) Plant Global Edu. Project, NAIST, Nara, Japan.

117
Identification and molecular characterisation of chloroplast ribonucleoprotein complexes involved in the second psaA-mRNA trans-splicing reaction. Olga Reifschneider1, Linnka Legendre-Lefebvre2, Christina Marx1, Jessica Jacobs1, Franziska Hundt3, Dirk Wolters3, Michel Goldschmidt-Clermont2, Ulrich Kück1. 1) Department for General and Molecular Botany, Ruhr-University Bochum, Germany; 2) Department of Botany and Plant Biology and Department of Molecular Biology, University of Geneva, Switzerland; 3) Department of Analytical Chemistry, Ruhr-University Bochum, Germany.


Circadian rhythm, cell cycle, and development

118
Putative Cdc25 phosphatases in the cell cycle regulation of Chlamydomonas reinhardtii. Monika Hlavová, Mária Čížková, Hana Pitrunová, Katerina Bisova. Laboratory of Cell Cycles of Algae, Inst Microbiology ASCR, Trebon, Czech Republic.

119
UV sensitivity variations in C.reinhardtii - a further investigation. Vishalsingh Chaudhari, Aniket Vyavahare, Swapan Bhattacharjee, Basuthkar Rao. Department of Biological Science, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India.

120
Cell-free extracts of C.reinhardtii exhibit excision repair activity against UVC damage in DNA. Vishalsingh Chaudhari, Vandana Raghavan, Basuthkar Rao. Department of Biological Science, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India.

121
Novel mutants affecting cell cycle regulation and DNA damage response in the green alga Chlamydomonas reinhardtii. Monika Hlavova, Maria Cizkova, Zoltan Turoczy, Katerina Bisova. Laboratory of Cell Cycles of Algae, Inst Microbiology AS CR, Třeboň, Czech Republic.

122
Promoters driving chloroplast gene expression in diurnal conditions. Adam Idoine, Alix Boulouis, Ralph Bock. Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.

123
Forward genetics study of the zygote differentiation mechanisms in Chlamydomonas reinhardtii. T. Kariwasam1, S. Joo1, D. Toor1, E. Cronmiller1, K. Noh2, T. Wulan2, U. Goodenough2, J.-H. Lee1. 1) Dept of Botany, Univ. of British Columbia, Vancouver, BC, Canada; 2) Dept. of Biology, Washington Univ., St.Louis, MO, USA.

124
Functional analysis of CRY-DASH1 in Chlamydomonas reinhardtii. Wei Li1, Lysett Wagner1, Sabine Oldemeyer2, Tilman Kottke2, Maria Mittag1. 1) Institute of General Botany and Plant Physiology, Friedrich Schiller University Jena, 07743 Jena, Germany; 2) Physical and Biophysical Chemistry, Department of Chemistry, Bielefeld University, 33615 Bielefeld, Germany.

125
Involvement of a putative SUMO peptidase in size-mediated cell cycle control in Chlamydomonas reinhardtii. Yang-Ling Lin, Chun-Han Chen, Su-Chiung Fang. Academia Sinica, SinShih Dist, Tainan, Taiwan.

126
Testing the constancy of the nuclear : cytoplasmic volume ratio in wild type and cell-size mutants of Chlamydomonas. D. Liu1, J. Umen2. 1) Washington University, St. Louis MO; 2) Donald Danforth Plant Science Center, St Louis MO.

127
Raptor and siRNA-mediated translation repression in Chlamydomonas reinhardtii. Tomohito Yamasaki1,2, Xinrong Ma1, Eun-Jeong Kim1, Heriberto Cerutti1. 1) School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska, Lincoln, Nebraska, USA; 2) Current address: School of Environmental Science and Engineering, Kochi University of Technology, Tosayamada, Kochi, Japan.

128
Effect of resource limitation on asexual development of Volvox carteri. Alexandra Harryman, Jose Ortega Escalante, Jacob Kott, Michael Ishak, Stephen Miller. Deptartment of Biological Sciences, UMBC, Baltimore, MD.

129
Actin-ring-independent cytokinesis in Chlamydomonas. Masayuki Onishi1, Luke Mackinder2, Martin Jonikas2, John R. Pringle1. 1) Dept of Genetics, Stanford University, Stanford, CA; 2) Dept of Plant Biology, Carnegie Institution for Science, Stanford, CA.

130
Key Protein Domain Functions in Assembly and Placement of the Eyespot. Mark Thompson, Telsa Mittelmeier, Eric Figueroa, Carol Dieckmann. MCB, University of Arizona, Tucson, AZ.

131
Functional analysis of an animal-like cryptochrome. Sandra Wenzel1, Benedikt Beel1, Tilman Kottke2, Maria Mittag1. 1) Institute of General Botany and Plant Physiology, Friedrich Schiller University, Am Planetarium 1, 07743 Jena, Germany; 2) Physical and Biophysical Chemistry, Department of Chemistry, Bielefeld University, Universitätsstraβe 25, 33615 Bielefeld, Germany.


Light, carbon, and other environmental responses

132
Predator induced aggregation of Chlamydomonas reinhardtii by a diffusible signal. Sarah Cossey1, Christopher Berger1, Nicole Richardson2, Bradley Olson1. 1) Kansas State University, Manhattan, KS; 2) University of Iowa, Iowa City, IA.

133
Chlamydomonas mutants that are hypersensitive to the TOR kinase inhibitor rapamycin show differential growth responses to carbon source. Inmaculada Couso-Lianez1, Spencer Diamond2, Garret Anderson3, Jia Li1, Jan Jaworski1, James Umen1. 1) Umen Lab, Donald Danforth Plant Science Center, St Louis, MO; 2) UCSD, La Jolla, CA 92093; 3) Salk Institute, La Jolla, CA 92037.

134
Characterization of NaCl stress inducible palmelloids in the green alga, Chlamydomonas reinhardtii. Jacinta D'Souza1, Dolly Khona1, Seema Shirolikar2, Erik Hom3, Manjushree Deodhar4. 1) UM-DAE Ctr for Excellence in Basic Sciences, Mumbai, Maharashtra; 2) 2Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India; 3) Department of Molecular and Cellular Biology, Harvard University, 520 Oxford Street, NW 469 Cambridge, Massachusetts 02138; 4) K. E. T’s V. G. Vaze College of Arts, Science and Commerce, Mulund (E), Mumbai 400 081, India.

135
Novel Retrograde Bilin Signaling Impacts Transition from Heterotrophic to Photoautotrophic Growth in Chlamydomonas reinhardtii. Deqiang Duanmu1, Nathan Rockwell1, David Casero2,3, Tyler Wittkopp4, Stefan Schmollinger5, Rachel Dent6, Matteo Pellegrini2,3, Krishna Niyogi6,7,8, Sabeeha Merchant2,5, Arthur Grossman4, Clark Lagarias1. 1) Molecular and Cellular Biology, University of California Davis; 2) Institute for Genomics and Proteomics, University of California, Los Angeles; 3) Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles; 4) Department of Plant Biology, Carnegie Institution for Science, Stanford; 5) Department of Chemistry and Biochemistry, University of California, Los Angeles; 6) Department of Plant and Microbial Biology, University of California, Berkeley; 7) Howard Hughes Medical Institute; 8) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley.

136
CO2-requiring mutants defective in CCM-induction generated by high frequency transformation with square electric pulses. Lyanyong Wang1, Takashi Yamano1, Masataka Kajikawa1, Masafumi Hirono2, Hiro Iguchi1, Hideya Fukuzawa1. 1) Grad Sch Biostudies, Kyoto Univ, Kyoto, Japan; 2) Grad Sch Science, Univ of Tokyo, Japan.

137
Impact of carbon nutrition on mechanisms of cellular iron homeostasis. Anne Glaesener1, Sabeeha Merchant1,2. 1) Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA; 2) Institute of Genomics and Proteomics, University of California Los Angeles, Los Angeles, CA.

138
Characterization of a Null Mutant of Isocitrate Lyase in Chlamydomonas. C. Plancke1, H. Vigeolas1, R. Höhner2, R. Willamme1, S. Roberty1, P. Cardol1, S. Hiligsmann1, P. Thonart1, G. Eppe1, M. Hippler1, U. Goodenough3, C. Remacle1. 1) University of Liege, Belgium; 2) University of Muenster, Germany; 3) Washington University, St. Louis, MO.

139
Biochemical characterization of "truncated" hemoglobins from Chlamydomonas reinhardtii. Dennis Huwald, Melis Düner, Thomas Happe, Anja Hemschemeier. Ruhr-University of Bochum, Bochum, NRW, Germany.

140
Assessing the use of genetically engineered microalgae for the bioremediation of toxic metals. Aniefon A. Ibuot, Jon K. Pittman. Faculty of Life Sciences,University of Manchester, UK.

141
Development of Chlamydomonas reinhardtii strains with improved photosynthetic activity and growth rate. Jooyeon Jeong, KwangRyul Beak, EonSeon Jin. Dept. of Life science, Hanyang University, Seoul, South Korea.

142
Towards Understanding Algal Truncated Hemoglobins – in vitro and in vivo Characterization of THB1. Eric A. Johnson, Selena L. Rice, Matthew R. Preimesberger, Juliette T. Lecomte. Biophysics, Johns Hopkins University, Baltimore, MD.

143
Identification of proteins involved in contractile vacuole function in Chlamydomonas reinhardtii using a transcriptome approach. Karin Komsic-Buchmann, Burkhard Becker. Botanical Institute, University of Cologne, Koeln, Germany.

144
Nutrient availability and growth conditions influence the accumulation of neutral lipids in eukaryotic algae. Michael McConnell1, Byeong-ryool Jeong1, Amanda Kobayashi1, Sean Awakuni1, Heriberto Cerutti2, Karin van Dijk1. 1) Department of Biology, Creighton University, Omaha, NE, USA; 2) School of Biological Sciences, University of Nebraska, Lincoln, NE USA.

145
The Chlamydomonas S-nitrosylome. S. Morisse1, M. Zaffagnini1,2, X. H. Gao1, C. H. Marchand1, S. D. Lemaire1. 1) Centre National de la Recherche Scientifique, Sorbonne Universités UPMC Univ Paris 06, UMR8226, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, Paris, France; 2) Laboratory of Plant Redox Biology, Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy.

146
Identification of potential components of the carbon concentrating mechanism in Chlamydomonas reinhardtii through insertional mutagenesis. Bratati Mukherjee, Marylou Machingura, Joanna Bajsa-Hirschel, Ananya Mukherjee, Susan Laborde, Nadine Jungnick, James Moroney, Dequantarius Speed, Julie Cronan. Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70802.

147
Comparative genomics in Chlamydomonas identifies an ancient nuclear envelope protein family essential for sexual reproduction in protists, fungi, plants, and vertebrates. Jue Ning1, Thomas Otto2, Claudia Pfander2, Frank Schwach2, Mathieu Brochet2, Ellen Bushell2, David Goulding2, Mandy Sanders2, Paul Lefebvre3, Jimin Pei4,5, Nick Grishin4,5, Gary Vanderlaan1, Oliver Billker2, William Snell1. 1) Department of Cell Biology, University of Texas Southwestern Medical School, Dallas; 2) Wellcome Trust Sanger Institute, Hinxton Cambridge CB10 1SA, United Kingdom; 3) Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108, USA; 4) Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA; 5) Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.

148
A broader redox network in Chlamydomonas reinhardtii revealed by revisiting the proteome of thioredoxin targets. M. E. Perez-Perez1, A. Mauries1, N. Tourasse2, S. D. Lemaire1, C. H. Marchand1. 1) Centre National de la Recherche Scientifique, Sorbonne Universités UPMC Univ Paris 06, UMR8226, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, 75005 Paris, France; 2) Centre National de la Recherche Scientifique, FRC550, Institut de Biologie Physico-Chimique, 75005 Paris, France.

149
The Chlamydomonas UVS9 gene encodes an XPG homolog vital for DNA repair. Erin Deitsch1, Erin Hibbard2, Jason Petersen3,4,5. 1) The Center for Biological Control and Analysis by Applied Photonics (BCAAP), South Dakota State University, Brookings, SD; 2) Vassar College, Poughkeepsie, NY; 3) Avera Institute for Human Genetics, Avera Research Institute, Sioux Falls, SD; 4) Sioux Falls VA Health Care System, Sioux Falls, SD; 5) Sanford School of Medicine of the University of South Dakota, Sioux Falls, SD.

150
Regulation of CCM genes in Chlamydomonas reinhardtii during conditions of light–dark cycles in synchronous cultures. Srikanth Tirumani1,2, Mallikarjuna Kokkanti2, Vishalsingh Chaudhari1, Manish Shukla1, Basuthkar Rao1. 1) Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, India; 2) Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, Andhra Pradesh, India.

151
Activation of the redox-regulated algal HSP33 that lost its Zn-binding activity during evolution. Na'ama Segal, Michal Shapira. Life Science, Ben-Gurion University of the Negev, Beer-Sheva , Israel.

152
Abiotic Stress Induced PCD through Mitochondrian - Caspase Protease Pathway in Chlamydomonas reinhardtii. Sirisha L. Vavilala, Mahuya Sinha, Jacinta D'Souza. Department of Biology, UM-DAE Centre for Excellence in Basic Sciences, Kalina Campus, Santacruz (E), Mumbai-400098, Maharashtra, India.


Biofuels

153
Trade-off between biomass production and Non-Photochemical-Quenching (NPQ) in Chlamydomonas reinhardtii. Silvia Berteotti, Matteo Ballottari, Roberto Bassi. Biotechnology, University of Verona, Verona, Verona, Italy.

154
Over-expression of the Calvin cycle enzyme FBPase in the Chlamydomonas reinhardtii chloroplast: effect on growth and biomass. W. Dejtisakdi, S. M. Miller. Department of Biological Sciences, UMBC, Baltimore, MD 21250 USA.

155
Bio-hydrogen production in the green algae Chlamydomonas: effect of H2 partial pressure and acetate. David Gonzalez-Ballester, Jose Luis Jurado-Oller, Aurora Galvan, Emilio Fernandez. Bioquimica y Biologia Molecular, Universidad de Cordoba, cordoba, Cordoba, Spain.

156
Comparison between nitrogen deprivation and high light as triggers for neutral lipid accumulation in Chlamydomonas reinhardtii. Hugh D. Goold1,2, Stephane Cuine2, Bertrand Legeret2, Patrick Carrier2, Pascaline Auroy2, Brian Jones1, Frederic Beisson2, Gilles Peltier2, Yonghua Li-Beisson2. 1) Faculty of Agriculture and the Environment, University of Sydney, Sydney, NSW, Australia; 2) CEA, CNRS, Aix-Marseille University, CEA Cadarache, Saint-Paul-lez-Durance, F-13108 France.

157
Enhancement of starch accumulation in Chlamydomonas reinhardtii mutated in a maltose transporter. S. Jang1, Y. Yamaoka1, H. Kim1, D. Ko2, J. Lee2, T. Kurita3, K. Kim1, Y. Kim1, W. Song1, I. Nishida3, Y. Lee1,2. 1) Department of Life Sciences, POSTECH, Pohang, South Korea; 2) Department of Integrative Bioscience & Biotechnology, POSTECH, Korea; 3) Division of Life Science, Graduate School of Science and Engineering, Saitama University, Japan.

158
The fungicide fenpropimorph induces conversion of plastidial membrane lipids to triacylglycerols in Chlamydomonas. H. Kim1, S. Jang1, S. Kim1, Y. Yamaoka1, D. Hong1, I. Nishida3, Y. Li-Beisson4, Y. Lee2. 1) Life science, POSTECH, Pohang, South Korea; 2) of Integrative Biology and Biotechnology, POSTECH, Pohang, South Korea; 3) Life Science, Graduate School of Science and Engineering, Saitama University, Saitama, Saitama, Japan; 4) Plant Biology and Environmental Microbiology, CEA-CNRS-Aix Marseille University, Saint-Paul-Lez-Durance, France.

159
Induction of Triacylglycerol Turnover Through the β-Oxidation Pathway in Chlamydomonas reinhardtii Following Nitrogen Repletion. Joseph Msanne. University of Nebraska, Lincoln, Lincoln, NE.

160
Vectors for expression of alkane pathway enzymes in the Chlamydomonas reinhardtii chloroplast. R. Park, W. Dejtisakdi, S. Miller. University of MD, Baltimore County, Baltimore, MD 21250.

161
Fine-tuning transgene expression for ´designer´ biofuels production: a bottom-up approach. Juan Rico1, Mark Scaife1, Katherine Helliwell1, Ginnie Nguyen1, Saul Purton2, Alison Smith1. 1) Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK; 2) Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK.

162
Determining Chlamydomonas TAG Yield Using a Commercial Serum Triglyceride Determination Kit. Taylor Weiss1, Carrie Goodson1, Jan Jaworski2, Jia Li2, Tuya Wulan1, Ursula Goodenough1. 1) Washington University, St. Louis, MO; 2) Danforth Plant Science Center, St. Louis, MO.


Metabolism (including hydrogen)

163
Suppression of the mutations in the gene CHLH encoding the large subunit of magnesium chelatase in Chlamydomonas reinhardtii. Elena M. Chekunova1, Elena B. Yaronskaya2, Natalia V. Yartseva1. 1) St.-Petersburg State University, Saint-Petersburg, Russian Federation; 2) Institute of Biophysics and cell engineering, National Academy of Sciences of Belarus, Republic of Belarus.

164
Functional differences between Chlamydomonas root-type FDX2 and leaf-type FDX1 may be attributed to a few amino acid residues based: link to H2 and NADPH photoproduction. Alexandra Dubini. national renewable energy laboratory, golden, CO.

165
Characterization of low-TAG accumulating mutant in Chlamydomonas reinhardtii. M. Kajikawa1, Y. Sawaragi1, H. Shinkawa1, T. Yamano1, M. Hirono2, N. Sato3, H. Fukuzawa1. 1) Grad. Sch. of Biostudies, Kyoto Univ., Japan; 2) Grad. Sch. of Sci, Univ. of Tokyo, Japan; 3) Grad. Sch. of Arts and Sci, Univ. of Tokyo, Japan.

166
Toward Mosquito Control with Green Algae: Expression of Cry Genes from Bacillus thuringiensis israelensis in the Chloroplast of Chlamydomonas . Seongjoon Kang, Obed W. Odom, David L. Herrin. Department of Molecular Biosciences, School of Biological Sciences, University of Texas at Austin, TX, 78712, USA.

167
Carbonate and acetate metabolisms in Chlamydomonas reinhardtii by NMR. J. Chang1, M. Singh2, C. Goodson3, N. van der Velde1, A. Nguyen1, U. Goodenough3, S. J. Kim1. 1) Chemistry, Baylor Univeristy, Waco, TX; 2) Chemistry, Washington University, St. Louis, MO; 3) Biology, Washington University, St. Louis, MO.

168
Bimodal C. reinhardtii Network Co-evolution Revealed by Integrated Phylogenomics and Metabolic Network Analyses. Kourosh Salehi-Ashtiani1, Amphun Chaiboonchoe1, Lila Ghamsari2, Bushra Dohai1, Patrick Ng3, Ashish Jaiswal1, Hong Cai1, David Nelson1, Xinping Yang2, Jason Papin4, Haiyuan Yu3, Santhanam Balaji5. 1) New York University Abu Dhabi, Abu Dhabi, United Arab Emirates; 2) Center for Cancer Systems Biology (CCSB) and Department of Cancer Biology, Dana-Farber Cancer Institute, and Department of Genetics, Harvard Medical School, Boston, MA, USA; 3) 3Department of Biological Statistics and Computational Biology and Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA; 4) Department of Biomedical, Engineering, University of Virginia, Charlottesville, VA, USA; 5) 5MRC Laboratory of Molecular Biology, Cambridge, UK.

169
Isolation and characterization of lipid-accumulating mutants under photoautotrophic conditions using flow cytometer. H. Shinkawa, M. Kajikawa, H. Fukuzawa. Grad. Sch. of Biostudies, Kyoto Univ., Japan.

170
Identification of the chloroplast D-lactate dehydrogenase involved in pyruvate reduction in Chlamydomonas reinhardtii. Hussein Taha1,2, Steven J. Burgess3, Justin A. Yeoman1, Oksana Iamshanova1, Peter J. Nixon1. 1) Department of Life Sciences, Imperial College London, London SW7 2AZ, UK; 2) Faculty of Sciences, Universiti Brunei Darussalam, Gadong BE1410, Brunei Darussalam; 3) Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK.

171
Acetate Production Pathways in Chlamydomonas reinhardtii during Dark Anoxia and the Dominant Role of Chloroplasts in Fermentative Acetate Production. Wenqiang Yang1, Claudia Claudia Catalanotti1, Sarah D’Adamo2, Tyler Wittkopp1,3, Cheryl Ingram-Smith4, Luke Mackinder1, Tarryn Miller2, Kerry Smith4, Martin Jonikas1, Arthur Grossman1, Matthew Posewitz2. 1) Plant Biology, Carnegie Institution for Science, Stanford, CA; 2) Colorado School of Mines, Golden, CO; 3) 3Stanford University, Stanford, CA; 4) Clemson University, Clemson, SC.


Beyond C. reinhardtii (Volvocales and other algae)

172
Evolution of a soma-determining gene. P. J. Ferris1, E. R. Hanschen1, Z. I. Grochau-Wright1, B. J. S. C. Olson2, R. E. Michod1. 1) Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ; 2) Division of Biology, Kansas State University, Manhattan, KS.

173
Discovery of regA Family Genes in non-Volvox Species. Z. Grochau-Wright1, P. Ferris1, E. Hanschen1, B. Olson2, R. Michod1. 1) Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ; 2) Division of Biology, Kansas State University, Manhattan, Kansas.

174
New selectable markers for Volvox carteri. Jose Ortega, Owen Kwok, Stephen Miller. Biological Science, UMBC, baltimore, MD.

175
Microbial consortia: A way forward for the commercialisation of microalgae for industrial biotechnology? C. J. A. Ridley, E. Kazamia, A. G. Smith. Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.

176
Symbiodinium Transcriptome and Global Responses to Changing Light Levels. Tingting Xiang1, William Nelson1,2, Jesse Rodriguez3, Dimitri Tolleter1,4, Arthur Grossman1. 1) Department for Plant Biology, Carnegie Institution for Science, Stanford, CA. 94305; 2) Maverix, 1670 South Amphlett Boulevard, Suite 214, San Mateo, CA 94402; 3) Stanford University, Biomedical Informatics Program, Stanford, CA 94305; 4) Division of Plant Science, Research School of Biology, The Australian National University, Canberra, ACT, Australia.


Workshop on emerging technologies

177
Developing the microdroplet platform for microalgal biotechnology. Roshni J. Best1,2, Jie Pan1, Chris Abell1, Alison G. Smith2. 1) Department of Chemistry, University of Cambridge, Cambridge, United Kingdom; 2) Department of Plant Sciences, University of Cambridge, Cambridge, United Kingdom.

178
Establishing CRISPR/Cas9 for Gene Targeting in Chlamydomonas. Andre Greiner, Lina Sciesielski, Peter Hegemann. Experimental Biophysics, Humboldt-University, Berlin, Berlin, Germany.

179
Rapid constructing and screening artificial microRNA systems in Chlamydomonas reinhardtii. Jinlu Hu1,2, Xuan Deng1, Gaohong Wang1, Kaiyao Huang1. 1) Key Laboratory of Algal Biology, Institute of Hydrobiology, C.A.S, Wuhan, Hubei, China; 2) University of the Chinese Academy of Sciences, Beijing 100039, China.

180
A genome-wide mutant library to transform Chlamydomonas research. Xiaobo Li, Weronika Patena, Ru Zhang, Spencer Gang, Sean Blum, Nina Ivanova, Rebecca Yue, Arthur Grossman, Martin Jonikas. Department of Plant Biology, Carnegie Institution for Science, Stanford, CA.

181
TALE activation of endogenous genes in Chlamydomonas reinhardtii. Han Gao1, David A. Wright1, Ting Li1, Yingjun Wang1, Kempton Horken2, Donald P. Weeks2, Bing Yang1, Martin H. Spalding1. 1) Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA; 2) Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE.


New directions in Chlamydomonas biology

182
Flagellar adhesion triggers shedding of a unique, SAG1-C65-containing flagellar ectosome compartment. Muqing Cao, Jue Ning, Carmen Hernandez-Lara, Olivier Belzile, William Snell. Cell Biology Department, UTSouthwestern Medical Center, Dallas, TX.

183
High Throuput Phenotyping of New Chlamydomonas Species Isolated from Musaffah, UAE and New York, USA. Amphun Chaiboonchoe, Hong Cai, Kelly Dougherty, Ashish Jaiswal, David R. Nelson, Marc Arnoux, Kourosh Salehi-Ashtiani. Division of Science and Math, New York University Abu Dhabi and Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi Institute, Abu Dhabi, UAE.

184
Flagellar waveform affects bioconvection behavior of Chlamydomonas reinhardtii. Azusa Kage, Yoshihiro Mogami. Ochanomizu University, Tokyo, Japan.

185
The Chlamydomonas genome encodes an active peptide processing enzyme. D. Kumar1, C. Blaby-Haas3, S. Merchant3, S. King1, R. Mains2, B. Eipper1. 1) Molecular Biology & Biophysics, Univ of Connecticut Health Center, Farmington, CT; 2) Dept of Neuroscience, Univ of Connecticut Health Center, Farmington, CT; 3) Dept of Chemistry & Biochemistry, Univ of California, LA.

186
Chlamydomonas Resource Center. P. Lefebvre, C. Silflow, M. Laudon. Plant Biology, University of Minnesota, St Paul, MN.

187
Why and how to exchange the type I RubisCO in Chlamydomonas reinhardtii with a bacterial type II RubisCO. Theresa Quaas, Anja Günther, Torsten Jakob, Christian Wilhelm. Institute of Biology, University of Leipzig, Johannisallee 23, 04103 Leipzig, Germany.