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2014 Important Dates

Abstract Submission Opens January 8
Conference Registration Opens January 15
Abstract Submission Deadline (extended) March 17
12 pm ET
Early Conference Registration  & Housing Deadline May 8
Abstract Revision Deadline (extended) March 18
Platform/Poster Assignments Online May 1

Platform Session Listing

 

 


Sunday, June 8   7:30 pm–9:00 pm
Merrill Hall

Perspective Lectures


Co-Chairs: Kris Niyogi, University of California, Berkeley and Winfield Sale, Emory University

Presentations:

1 - 7:30
Motility and more: the flagellum of African trypanosomes. Kent L. Hill. MIMG, UCLA, Los Angeles, CA.

2 - 8:15
Microalgal glycerolipid metabolism in the context of cell development and cell division. C. Benning. Michigan State University, East Lansing, MI.


Monday, June 9   8:30 am–10:00 am
Merrill Hall

Emerging Technologies


Chair: Sabeeha Merchant, University of California, Los Angeles

Presentations:

3 - 8:30
Chlamy in the light: technologies for today’s discoveries. Sabeeha S. Merchant. Institute for Genomics and Proteomics, UCLA, Los Angeles, CA.

4 - 8:45
Genomic Resequencing of Chlamydomonas Laboratory Strains Reveals Corrected Phylogeny and Identifies Variant Genes. Sean D. Gallaher1, Sorel T. Fitz-Gibbon2, Sabeeha S. Merchant1. 1) Chem & Biochem, UCLA, Los Angeles, CA; 2) MCDB, UCLA, Los Angeles, CA.

5 - 9:00
Using whole genome sequencing to identify mutations in Chlamydomonas. Huawen Lin, David Granas, Alison Albee, Paul Cliften, Susan Dutcher. Genetics, Washington Univ Sch Med, St Louis, MO.

6 - 9:15
High-throughput genotyping of Chlamydomonas mutants reveals random distribution of mutagenic insertion sites and endonucleolytic cleavage of transforming DNA. Weronika Patena*, Ru Zhang*, Ute Armbruster, Spencer Gang, Sean Blum, Martin Jonikas. Plant Biology, Carnegie Institution for Science, Stanford, CA.

7 - 9:30
The Functional annotator tool. T. Mühlhaus, A. Lüdemann, M. Schroda. Molecular Biotechnology & Systems Biology, TU Kaiserslautern, Kaiserslautern, Rheinland Pfalz, Germany.

8 - 9:45
Analysis of the transgene expression potential of Chlamydomonas expression strains. Rouhollah Barahimipour, Juliane Neupert, Ralph Bock. Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.


Monday, June 9   10:30 am–12:00 noon
Merrill Hall

Assembly of the Flagellar Axoneme


Chair: Susan Dutcher, Washington University School of Medicine

Presentations:

9 - 10:30
A mutation in cullin, a scaffold for the ubiquitin E3 ligase, suppresses missense mutations in cytoplasmic dynein. Susan Dutcher, Nicholas Nauman, Katherine Ferkol, Huawen Lin. Genetics, Washington Univ Sch Med, St Louis, MO.

10 - 10:45
Functional analysis of the outer arm dynein assembly factors CCDC103 and FBB18/C21ORF59. Stephen King, Ramila Patel-King. Molecular Biology and Biophysics, Univ Connecticut Health Ctr, Farmington, CT.

11 - 11:00
Novel properties of radial spoke assembly at the proximal axoneme. Lea M. Alford1, Alexa L. Matteyses1, Emily L. Hunter1, Huawen Lin2, Susan K. Dutcher2, Winfield S. Sale1. 1) Cell Biology, Emory University. 465 Whitehead Building, 615 Michael St., Atlanta, GA 30322; 2) Department of Genetics, Washington University School of Medicine. Box 8232, 660 S. Euclid Ave., St. Louis, MO 63110.

12 - 11:15
Direct Observation of IFT Turnover at the Flagellar Tip and Base using the Photobleaching Gate Assay. Ahmet Yildiz. Molecular Cell Biology, University of California Berkeley, Berkeley, CA.

13 - 11:30
Proteomic and single molecule studies on the role of D1bLIC in retrograde IFT and flagellar assembly. A. Schauer, J. Reck, K. VanderWaal, R. Bower, D. Tritschler, C. Perrone, M. Porter. GCD, University of Minnesota, Minneapolis, MN.

14 - 11:45
The ciliary inner dynein arm, I1 preassembles in the cytoplasm and requires IFT for transport in the cilium. Rasagnya Viswanadha1, Emily Hunter1, Ryosuke Yamamoto1, Lea Alford1, Maureen Wirschell2, Winfield Sale1. 1) Cell Biology, Emory University, Atlanta, GA; 2) Biochemistry, University of Mississippi, Jackson, MS.


Monday, June 9   1:15 pm–4:00 pm
Merrill Hall

Light, Carbon, and other Environmental Responses


Chair: Michael Schroda, University of Kaiserslautern

Presentations:

15 - 1:15
How Chlamydomonas responds to heat stress - a top-down Systems Biology approach. D. Hemme1,2, D. Veyel2, T. Mühlhaus1,2, F. Sommer1,2, J. Jüppner1,2, A. Unger3, M. Sandmann4, I. Fehrle2, S. Schönfelder2, M. Steup4, S. Geimer3, J. Kopka2, P. Giavalisco2, M. Schroda1,2. 1) Molecular Biotechnology & Systems Biology, TU Kaiserslautern, Kaiserslautern, Germany; 2) Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany; 3) Biology/Electron Microscopy, University of Bayreuth, Bayreuth, Germany; 4) Institute of Biochemistry und Biology, University of Potsdam, Potsdam-Golm, Germany.

16 - 1:30
Hypoxia signaling in Chlamydomonas. Melis Düner, Dennis Huwald, Thomas Happe, Anja Hemschemeier. Dept Photobiotechnology, Ruhr-University of Bochum, Bochum, NRW, Germany.

17 - 1:45
Phosphoprotein SAK1 is a regulator of acclimation to singlet oxygen in Chlamydomonas reinhardtii. Setsuko Wakao1, Brian Chin1, Heidi Ledford1, Rachel Dent1, David Casero2, Matteo Pellegrini2, Sabeeha Merchant3, Krishna Niyogi1,4. 1) Plant and Microbial Biology, University of California, Berkeley, CA 94720; 2) Molecular, Cell, and Developmental Biology, University of California, Los Angeles, CA 90095; 3) Chemistry and Biochemistry, University of California, Los Angeles, CA 90095; 4) Howard Hughes Medical Institute.

18 - 2:00
Insights into ROS signal transduction pathways from algae to higher plants. N. Shao1,2, G. Duan1, R. Bock1. 1) Max-Planck-Institut für Molekulare Pflanzenphysiologie, 14476 Potsdam-Golm, Germany; 2) Plant Gene Engineering Center , South China Botanical Garden, Chinese Academy of Sciences, Guang Zhou ,Guangzhou 510650, China.

19 - 2:15
Safety in Numbers: The Molecular Basis of Predator Evasion Behavior in Chlamydomonas reinhardtii. Bradley Olson, Christopher Berger, Sarah Cossey, Nicole Richardson. Div Biology, Kansas State Univ, Manhattan, KS.

2:30 pm - Break

20 - 2:45
Nuclear proteome dynamics in acclimation to stress. L. Magneschi1,2, R. Bayersdorf1, D. Nikolova1, C. Fufezan1, M. Hippler1. 1) Institute of Plant Biology and Biotechnology, University of Muenster, Muenster, Germany; 2) Alexander von Humboldt Stiftung/Foundation, Jean-Paul-Str. 12, 53173 Bonn, Germany.

21 - 3:00
HLA3 and LCIA are associated with inorganic carbon transport in Chlamydomonas reinhardtii. Takashi Yamano, Emi Sato, Hiro Iguchi, Hideya Fukuzawa. Graduate School of Biostudies, Kyoto Univ, Kyoto, Japan.

22 - 3:15
Two Differentiated Quiescent Cell Types of C. reinhardtii. Jannette Rusch, Robyn Roth, Tuya Wulan, Ursula Goodenough. Washington University, St. Louis, MO.

23 - 3:30
Systems analysis of nitrogen sparing mechanisms in Chlamydomonas. Stefan Schmollinger1, Timo Mühlhaus2,3, Nanette R. Boyle1, Ian K. Blaby1, David Casero4,6, Tabea Mettler3, Jeffrey L. Moseley5, Janette Kropat1, Frederik Sommer2,3, Daniela Strenkert1, Dorothea Hemme2,3, Arthur R. Grossman5, Mark Stitt3, Matteo Pellegrini4, Michael Schroda2,3, Sabeeha S. Merchant1,6. 1) Department of Chemistry and Biochemistry, UCLA; 2) Molecular Biotechnology and Systems Biology, TU Kaiserslautern; 3) Max Planck Institute of Molecular Plant Physiology, Potsdam; 4) Department of Molecular, Cell, and Developmental Biology, UCLA; 5) Department of Plant Biology, Carnegie Institution for Science; 6) Institute of Genomics and Proteomics, UCLA.

24 - 3:45
Acclimation responses of Chlamydomonas reinhardtii to sulfur deficiency. Munevver Aksoy, Arthur Grossman. Department of Plant Biology, Carnegie Institution for Science, Stanford, CA.


Monday, June 9   7:15 pm–9:00 pm
Merrill Hall

Circadian Rhythm, Cell Cycle, and Development


Chair: James Umen, Donald Danforth Plant Science Center

Presentations:

25 - 7:15
Identification of a Sizer Protein in Chlamydomonas. Y. Li1,2, B. J. S. C. Olson1,3, C. Lopez-Paz4, D. Liu4,5, G. Anderson1, J. Umen4. 1) Plant Biology Laboratory, Salk Institute, La Jolla, CA; 2) Dept of Horticultural and Plant Sci, Univ of Fla, Gainesville, FL; 3) Division of Biology, Kansas State Univ, Manhattan, KS; 4) Donald Danforth Plant Sci Ctr, St Louis, MO; 5) Plant and Microbial Biosci Prog, Wash Univ, St Louis, MO.

26 - 7:30
Genetic characterization of Chlamydomonas cell cycle control. Frej Tulin, Frederick Cross. Rockefeller University, New York, NY.

27 - 7:45
Defects in a new class of sulfate/anion transporter links sulfur acclimation responses to intracellular glutathione levels and cell cycle control. Su-Chiung Fang1, Chin-Lin Chung1, Chun-Han Chen1, James Umen2. 1) Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan, Taiwan; 2) Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA.

28 - 8:00
Transcriptional profiling of the Chlamydomonas cell cycle. James Matthew Zones1,2, Sabeeha Merchant3, James G. Umen1. 1) The Donald Danforth Plant Science Center, St. Louis, MO, 63132; 2) Department of Biology, University of California San Diego, CA 92093; 3) Department of Chemistry and Biochemistry, University of California Los Angeles, CA 90095.

29 - 8:15
Coexpression analysis of a synchronized day/night cycling culture predicts isoform localization. Ian Blaby1, James Zones2, Sabeeha Merchant1,3, James Umen2. 1) Department of Chemistry and Biochemistry, University of California Los Angeles, CA 90095; 2) Donald Danforth Plant Science Center, St. Louis, MO 63132; 3) Institute of Genomics and Proteomics, University of California Los Angeles, CA 90095.

30 - 8:30
Dissection of the functional architecture of Chlamydomonas HAP2, the protein essential for gamete membrane fusion in plants and protists. Yanjie Liu, William Snell. Department of Cell Biology, UT-Southwestern Medical Center, Dallas, TX.

31 - 8:45
Genome-wide analysis of gene regulatory networks in early zygote development of Chlamydomonas reinhardtii. S. Joo1, Y. Nishimura2, M.-H. Wang1, E. Cronmiller1, J.-H. Lee1. 1) Dept of Botany, University of British Columbia, Vancouver, BC, Canada; 2) Dept of Botany, Kyoto University, Kita-Shirakawa, Kyoto, Japan.


Tuesday, June 10   8:30 am–9:45 am
Merrill Hall

Biofuels


Chair: Chia-Lin Wei, Joint Genome Institute

Presentations:

32 - 8:30
Epigenetic interrogation of the algal lipid biosynthetic pathway. Chia-Lin Wei. DOE Joint Genome Institute, Walnut Creek, CA.

33 - 8:45
Dual specificity tyrosine-phosphorylation-regulated kinase DYRKP-1 negatively regulates starch and oil accumulation during nutrient deprivation. Miriam Schulz-Raffelt1, Vincent Chochois1, Pascaline Auroy1, Emmanuelle Billon1, David Dauvillée2, Yonghua Li-Beisson1, Gilles Peltier1. 1) CEA, CNRS, Aix-Marseille Université, Institut de Biologie Environnementale et Biotechnologie, CEA Cadarache, F-13108 Saint-Paul-lez-Durance, France; 2) Unité de Glycobiologie Structurale et Fonctionnelle CNRS UMR 8576, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.

34 - 9:00
Substrate specificity and positional preference of Chlamydomonas diacylglycerol acyltransferases critical for stress-related biosynthesis of triacylglycerol. Jin Liu1,2, Danxiang Han3, Kangsup Yoon4, Qiang Hu4, Yantao Li1,2. 1) Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD, 21202 USA; 2) Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD, 21202 USA; 3) Department of Applied Sciences and Mathematics, Arizona State University Polytechnic campus, Mesa, AZ, USA; 4) Center for Microalgal Biotechnology and Biofuels, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China.

35 - 9:15
The Path to Triacylglycerol (TAG) Obesity in the sta6 Strain of Chlamydomonas reinhardtii. Ursula Goodenough1, Jae-Hyeok Lee2, Sabeeha Merchant3, James Umen4. 1) Washington University, St. Louis, MO; 2) University of British Colombia, Vancouver, BC; 3) University of California, Los Angeles, CA; 4) Danforth Plant Science Center, St. Louis, MO.

36 - 9:30
Towards a new bioenergetic concept: Increasing the photorespiratory glycolate excretion in C. reinhardtii for bio-methane production. Anja Guenther1, Theresa Quaas1, Susann Reinert1, Susanne Heithoff3, Mark Fresewinkel2, Torsten Jakob1, Christian Wilhelm1. 1) University of Leipzig, Institute of Biology, Johannisallee 23, 04103 Leipzig, Germany; 2) KIT, Institute of Life Science Engineering, Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany; 3) University of Bremen, Institute for Environmental Process Engineering, Leobener Str. 33, 28359 Bremen, Germany.


Tuesday, June 10   10:15 am–12:00 noon
Merrill Hall

Flagellar Length Regulation, Basal Bodies, and Transition Zone


Chair: Carolyn Silflow, University of Minnesota

Presentations:

37 - 10:15
Characterization of the Uni1 protein and its localization on basal bodies. M. LaVoie1, J. Salisbury2, P. Ranum1, P. Lefebvre1, C. Silflow1. 1) Dept. Plant Biology, University of Minnesota, St. Paul, MN; 2) Dept. Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.

38 - 10:30
Using Chlamydomonas to elucidate the structure and function of the ciliary transition zone. B. Craige, J. Awata, J. M. Brown, Y. Hou, G. B. Witman. UMass Medical School, Dept. of Cell and Developmental Biology, Worcester, MA.

39 - 10:45
The role of LF5 localization in the regulation of flagellar length. L.-W. Tam, P. Ranum, P. Lefebvre. Dept Plant Biol, Univ Minnesota, St Paul, MN.

40 - 11:00
A CDK-like protein kinase is a key regulator of flagellar disassembly. Z. Hu, Y. Liang, J. Pan. School of Life Sciences, #413 Renhuanlou, Tsinghua University, Beijing, Beijing, China.

41 - 11:15
IFT74 is required for IFT-A / IFT-B interaction, but not for import of tubulin into flagella. Jason M. Brown, Deborah A. Cochran, Tomohiro Kubo, George B. Witman. Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, MA.

42 - 11:30
IFT-dependent and IFT-independent transport of tubulin in Chlamydomonas flagella. Julie Craft, Karl Lechtreck. Cellular Biology, University of Georgia, Athens, GA.

43 - 11:45
Comparative proteomic analysis of vesicles released from flagellar membrane with isolated flagellar membranes. Huan Long1, Fan Zhang1, Dennis Diener2, Joel Rosenbaum2, Kaiyao Huang1. 1) Key Laboratory of Algal Biology, Institute of Hydrobiology, C.A.S, Wuhan, Hubei, China; 2) 2.Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520.


Tuesday, June 10   7:15 pm–9:00 pm
Merrill Hall

Photosynthesis


Chair: Jun Minagawa, National Institute for Basic Biology

Presentations:

44 - 7:15
Chloroplast remodeling during state transitions in Chlamydomonas reinhardtii as revealed by non-invasive techniques in vivo. Gergely Nagy1,2,3, Renáta Ünnep2, Ottó Zsiros4, Ryutaro Tokutsu5,6, Kenji Takizawa5, Lionel Porcar3, Lucas Moyet7,8,9,10, Dimitris Petroutsos7,8,9,10, Győző Garab4, Giovanni Finazzi7,8,9,10, Jun Minagawa5,6. 1) Paul Scherrer Institute, Laboratory for Neutron Scattering, 5232 Villigen PSI, Switzerland; 2) Wigner Research Centre for Physics, Institute for Solid State Physics and Optics, Hungarian Academy of Sciences; 3) Institut Laue-Langevin, BP 156, F-38042, Grenoble Cedex 9, France; 4) Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, POB 521, H-6701, Szeged, Hungary; 5) National Institute for Basic Biology (NIBB), Nishigonaka 38, Myodaiji, Okazaki 444-8585, Japan; 6) CREST, Japan Science and Technology Agency, Chiyoda-ku, Tokyo 102-0076, Japan; 7) Centre National de la Recherche Scientifique, UMR 5168 Laboratoire de Physiologie Cellulaire et Végétale; 8) Université Grenoble Alpes; 9) Institut National Recherche Agronomique (INRA); 10) Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Institut de Recherche en Sciences et Technologie du Vivant (IRTSV), CEA Grenoble, F-38054 Grenoble, France.

45 - 7:30
Light harvesting in Chlamydomonas reinhardtii - between adaptation and acclimation. Bartlomieij Drop1, Sathish Yadav2, Caner Unlu3, Egbert Boekema2, Herbert van Amerongen3, Roberta Croce1. 1) VU University Amsterdam, Amsterdam, the Netherlands; 2) University of Groningen, Groningen, the Netherlands; 3) Wageningen University, Wageningen, the Netherlands.

46 - 7:45
Phosphorylation dependent reorganization of photosynthetic multiprotein complexes. Sonja Verena Bergner, Martin Scholz, Kerstin Trompelt, Philipp Gäbelein, Johannes Barth, Christian Fufezan, Michael Hippler. Institute of Plant Biology and Biotechnology, University of Münster, Münster 48143, Germany.

47 - 8:00
Photoprotection in Chlamydomonas revisited: role of light and metabolism. D. Petroutsos1, R. Tokutsu2, S. Flori1, D. Karageorgou1, A. Greiner3, M. Mittag4, P. Hegemann3, J. Minagawa2, G. Finazzi1. 1) Cell and Plant Physiology Laboratory, CEA Grenoble, France; 2) Division of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Japan; 3) Institute for Experimental Biophysics, Humboldt University, Berlin, Germany; 4) Institute of General Botany and Plant Physiology, Friedrich Schiller University, Jena, Germany.

48 - 8:15
Identifying a Novel Type of Violaxanthin De-epoxidase from Chlamydomonas reinhardtii . Zhirong Li1, Rachel M. Dent1, Graham Peers2, Wiebke Apel1, Scarlett Yang1, Krishna K. Niyogi1,3. 1) Howard Hughes Medical Institute, Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA; 2) Department of Biology, Colorado State University, Fort Collins, Colorado 80523, USA; 3) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720-3102, USA.

49 - 8:30
Functional analysis of PBC1, a protein conserved in the green lineage that is associated with the PSII core and involved in high light adaptation. Ligia S. Muranaka1, Mark Rütgers1, Sandrine Bujaldon2, Anja Heublein3, Frederik Sommer1, Torsten Möhlmann4, Ekkehard Neuhaus4, Stefan Geimer3, Fabrice Rappaport2, Francis-André Wollman2, Michael Schroda1. 1) Molekulare Biotechnologie & Systembiologie, TU Kaiserslautern, Germany; 2) Institut de Biologie Physico-Chimique, Unité Mixte de Recherche 7141, Centre National de la Recherche Scientifique/ Université Pierre et Marie Curie, Paris, France; 3) Zellbiologie/Elektronenmikroskopie, Universität Bayreuth, Germany; 4) Pflanzenphysiologie, TU Kaiserslautern, Germany.

50 - 8:45
Comprehensive Identification of Genes Responsible for Photosynthesis and the Carbon Concentrating Mechanism of Chlamydomonas reinhardtii. Leif Pallesen, Greg Reeves, Weronika Patena, Saman Parsa, Rachel Purdon, Martin Jonikas. Department of Plant Biology, Carnegie Institution for Science, Stanford, CA.


Wednesday, June 11   8:30 am–10:00 am
Merrill Hall

Chloroplast Biogenesis and Function


Chair: William Zerges, Concordia University

Presentations:

51 - 8:30
Past, present, and potential roles of Chlamydomonas research in chloroplast biology. William Zerges, Uniacke James, Marco Schottkowski, Matthew Peters, Yi Sun, James Dhaliwal, Yu Zhan. Biology Department, Concordia University, Montreal, Quebec, Canada.

52 - 8:45
Interconnection between the chloroplast HSP70B chaperone system, VIPP1 and the Secretory Pathway in biogenesis of thylakoid membranes in Chlamydomonas. Mark Rütgers1, Lígia Muranaka1, Mareike Possienke2, Karolin Dorn2, Anja Heublein3, Stefan Geimer3, Michael Schroda1. 1) Molekulare Biotechnologie & Systembiologie, TU Kaiserslautern, Germany; 2) Institute of Biology II / Plant Biochemistry, University of Freiburg, Germany; 3) Zellbiologie/Elektronenmikroskopie, Universität Bayreuth, Germany.

53 - 9:00
Role of a novel SEC14 domain-containing protein in chloroplast lipid trafficking and photoautotrophic growth in Chlamydomonas reinhardtii. Jose Gines Garcia Cerdan1, Alizée Malnoë1,2, Alexander P. Hertle1,2, Krishna K. Niyogi1,2. 1) UC Berkeley-HHMI, Berkeley, CA; 2) Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

54 - 9:15
Intron tscA RNA is required for assembly of a chloroplast-splicing complex. Christina Marx1, Olga Reifschneider1, Jessica Jacobs1, Franziska Hundt2, Dirk Wolters2, Ulrich Kück1. 1) General and Molecular Botany, Ruhr-University Bochum, Bochum, Germany; 2) Analytical Chemistry, Ruhr-University Bochum, Bochum, Germany.

55 - 9:30
Nitric Oxide-triggered remodelling of chloroplast bioenergetics and thylakoid proteins upon nitrogen starvation in Chlamydomonas reinhardtii. Yves Choquet1, Lili Wei1, Benoit Derrien1, Arnaud Gautier2, Laura Houilles-Vernes1, Alix Boulouis1, Denis Saint-Marcoux1, Alizée Malnoë1, Fabrice Rappaport1, Catherine De Vitry1, Olivier Vallon1, Francis-André Wollman1. 1) UMR 7141, Inst Biologie Physico-Chimique, Paris, France; 2) UMR 8640, École Normale Supérieure, Département de Chimie, Paris, France.

56 - 9:45
Conditional depletion of the chloroplast ClpP1 protein activates nuclear genes involved in autophagy and chloroplast protein quality control. Silvia Ramundo1,2, David Casero3, Timo Mühlhaus4, Dorothea Hemme4, Frederik Sommer4, Michèle Crèvecoeur2, Michèle Rahire2, Michael Schroda4, Jannette Rusch5, Ursula Goodenough5, Matteo Pellegrini6, Maria Esther Perez-Perez7, José Luis Crespo7, Olivier Schaad8, Natacha Civic8, Martin Jonikas9, Peter Walter1, Jean David Rochaix2. 1) Department of Biohemistry and Biophysics, University of California, San Francisco, CA; 2) Departments of Molecular Biology and Plant Biology, University of Geneva, Geneva, Switzerland; 3) Institute for Genomics and Proteomics, University of California, Los Angeles, CA; 4) Max Planck Institute of Molecular Plant Physiology, Potsdam-Golm, Germany; 5) Department of Biology, Washington University, St Louis, MO; 6) Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA; 7) Instituto de Bioquimica Vegetal y Fotosintesis, CSIC-Universidad de Sevilla, Sevilla, Spain; 8) Genomics Platform, University of Geneva, Geneva, Switzerland; 9) Department of Plant Biology, Carnegie Institution for Science, Palo Alto, CA.


Wednesday, June 11   10:30 am–12:00 noon
Merrill Hall

Metabolism (including Hydrogen)


Chair: Arthur Grossman, Carnegie Institution for Science

Presentations:

57 - 10:30
The metabolic face of Chlamydomonas in the dark. Arthur Grossman1, Wenqiang Yang1, Claudia Catalanotti1, Tyler Wittkopp1,2, Sarah Sarah D’Adamo3, Matthew Posewitz3. 1) Department of Plant Biology, Carnegie Institution for Science, Stanford, CA; 2) Stanford University, Department of Biology, Stanford, CA; 3) Colorado School of Mines, Department of Chemistry and Geochemistry, Golden, CO.

58 - 10:45
Application of Phenotype Microarray to Improve Metabolic Network Modeling of Chlamydomonas reinhardtii. H. Cai, A. Chaiboonchoe, B. Dohai, D. Nelson, A. Jaiswal, K. Salehi-Ashtiani. New York University Abu Dhabi, Division of Science and Math, and New York University Abu Dhabi Institute, Center for Genomics and Systems Biology; Abu Dhabi, UAE.

59 - 11:00
Identification of mutants with constitutive HYDA1 expression using a motility screen. X. Sun, P. Lefebvre, M. LaVoie, C. Silflow. Dept. Plant Biology, University of Minnesota, St. Paul, MN.

60 - 11:15
The role of pyruvate-ferredoxin-oxidoreductase in Chlamydomonas reinhardtii fermentative metabolism. C. Catalanotti1, W. Yang1, S. D’Adamo2, A. Atteia3, R. van Lis3, W. Inwood4, M. Kobayashi4, K. Niyogi44, M. C. Posewitz2, A. R. Grossman1. 1) Carnegie Institution, Stanford, CA; 2) Colorado School of Mines, Golden, CO; 3) Centre National de la Recherche Scientifique-Aix-Marseille Université, France; 4) University of California, Berkeley.

61 - 11:30
Discovery of a novel Complex I biogenesis factor regulating mitochondrial gene expression. Nitya Subrahmanian1, Claire Remacle2, Patrice Hamel1. 1) Department of Molecular Genetics, Ohio State University, 500 Aronoff Laboratory, 318 W. 12th avenue, 43210 Columbus, Ohio,; 2) Genetics of Microorganisms Laboratory, Department of Life Sciences, Université de Liège, B-4000, Liège, Belgium.

62 - 11:45
Exploiting thiamine regulation in Chlamydomonas for novel regulatory tools. Ginnie T. D. T. Nguyen, Mark A. Scaife, Chloë E. Scott, Alison G. Smith. Department of Plant Sciences, University of Cambridge, Cambridge, UK, CB2 3EA.


Wednesday, June 11   1:15 pm–4:00 pm
Merrill Hall

New directions in Chlamydomonas biology


Chair: José Luis Crespo, CSIC

Presentations:

63 - 1:15
Control of ER stress-induced autophagy by ROS in Chlamydomonas. Marta Pérez-Martín1, María Esther Pérez-Pérez2, Stéphane D. Lemaire2, José L. Crespo1. 1) Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC-Universidad de Sevilla, 41092 Sevilla, Spain; 2) Centre National de la Recherche Scientifique, Sorbonne Universités UPMC Univ. Paris 06, UMR8226, Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes, Institut de Biologie Physico-Chimique, 75005 Paris, France.

64 - 1:30
Chlamydomonas Argonaute3 is the major catalytic engine of miRNA-mediated post-transcriptional gene silencing. Tomohito Yamasaki, Takeshi Ohama. Department of Environmental Systems Engineering, Kochi Univ of Tech, Kochi, Japan.

65 - 1:45
Computational prediction of AGO3 associated microRNAs and their targets in Chlamydomonas reinhardtii. Adam Voshall1, Eun-Jeong Kim1, Etsuko N. Moriyama1,2, Heriberto Cerutti1,2. 1) School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE; 2) School of Biological Sciences and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE.

66 - 2:00
Discovery of long non-coding RNAs via their distinct chromatin signature in Chlamydomonas. D. Strenkert1, S. Cokus1, SD Gallaher1, JM Zones2, JG Umen2, M. Pellegrini1, SS Merchant1. 1) Institute of Genomics and Proteomics, University of California, Los Angeles; 2) Donald Danforth Plant Science Center St. Louis, Missouri.

67 - 2:15
A SUMO E2 conjugase mutant in Chlamydomonas reinhardtii reveals the role of SUMOylation during stress responses. Amy Knobbe1, Kempton Horken1, Thomas Plucinak1, Eniko Balassa2, Heriberto Cerutti2, Donald Weeks1. 1) Dept Biochemistry, Univ Nebraska, Lincoln, NE; 2) School of Biol. Sci., Univ Nebraska, Lincoln, NE.

2:30 pm - Break

68 - 2:45
Systems Biology in Chlamydomonas reinhardtii: A Case Study Using Contractile Vacuole Function and Osmoregulation as Example. Burkhard Becker, Karin Komsic-Buchmann. Biozentrum Köln, Universität zu Köln, Köln, Germany.

69 - 3:00
Evolution of sex in Chlamydomonas as a response to grazing. Hanna Koch, Martha Valiadi, Lutz Becks. Evolutionary Ecology & Community Dynamics, Max Planck Institute for Evolutionary Biology, Plön, Schleswig-Holstein, Germany.

70 - 3:15
Interkingdom Signaling Between Chlamydomonas reinhardtii and Bacteria. Sathish Rajamani1, Max Teplitski2, Richard Sayre1. 1) Bio-Labs, New Mexico Consortium, Los Alamos, NM, USA; 2) Department of Soil and Water Sciences, University of Florida, Gainesville, FL, USA.

71 - 3:30
RNA is a component of Chlamydomonas flagella and Tetrahymena cilia. P. Lefebvre1, P. Ranum1, L.-W. Tam1, W. Dentler2. 1) Dept Plant Biol, Univ Minnesota, St Paul, MN; 2) Dept Molecular Bioscience, Univ Kansas, Lawrence, KS.


Thursday, June 12   8:30 am–9:45 am
Merrill Hall

Beyond C. reinhardtii (Volvocales and other Algae)


Chair: Rachael Morgan-Kiss, Miami University

Presentations:

72 - 8:30
The Antarctic Chlamydomonas raudensis: a case for advances in understanding photosynthetic stress adaptation in non-model organisms. Rachael Morgan-Kiss1, Sarah Stahl1, Wei Li1, Jenna Dolhi1, Andor Kiss1,2. 1) Microbiology, Miami University, Oxford, OH; 2) Center for Bioinformatics & Functional Genomics, Miami University, Oxford, OH.

73 - 8:45
An emerging unicellular green alga model system for studying regulation of photosynthesis. Melissa Roth1, Sean Gallaher2, Shawn Cokus3, David Lopez3, Matteo Pellegrini3, Sabeeha Merchant2, Krishna Niyogi1,4. 1) Plant and Microbial Biology, UC Berkeley; 2) Department of Chemistry and Biochemistry, UCLA; 3) Department of Molecular, Cell and Developmental Biology, UCLA; 4) Howard Hughes Medical Institute.

74 - 9:00
Body size, offspring number, and generation time in volvocine algae: regulation of interrelated traits during a major evolutionary transition. Deborah E. Shelton, Richard E. Michod. Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ.

75 - 9:15
Evolution of cellular mechanisms of embryo inversion that enabled volvocine algae to transition from flat to spherical colony. P. R. V. Elvira1, I. Nishii1,2. 1) Temasek Life Sciences Laboratory, Singapore; 2) Department of Biological Sciences, NUS, Singapore.

76 - 9:30
The Genome of Gonium pectorale: Early evolutionary co-option of genes important for multicellularity occurred during the transition to colonial multicellularity. Bradley Olson1, Erik Hanschen2, Tara Marriage1, Patrick Ferris2, Takashi Hamaji3, Hisayoshi Nozaki3, Atsushi Toyoda4, Asao Fuiyama4, Pierre Durand5, David Smith6, Richard Michod2. 1) Div Biology, Kansas State Univ, Manhattan, KS; 2) Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ; 3) University of Tokyo, Tokyo, Japan; 4) National Institute of Genetics, Japan; 5) University of Witswaterstrand, Johannasberg, South Africa; 6) University of Western Ontario, Canada.


Thursday, June 12   10:15 am–12:00 noon
Merrill Hall

Mechanism and Regulation of Flagellar Motility


Chair: David Mitchell, SUNY Upstate Medical University

Presentations:

77 - 10:15
Axonemal dynein assembly mechanisms. Anudariya Dean, Paurav Desai, Judy Freshour, David Mitchell. Dept Cell & Dev Biol, SUNY Upstate Med Univ, Syracuse, NY.

78 - 10:30
Breaststroke flagellar photoresponse in Chlamydomonas reinhardtii. Kyriacos C. Leptos, Kirsty Y. Wan, Raymond E. Goldstein. Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge, United Kingdom.

79 - 10:45
Outer Dynein Arm activity is modulated by ethanol. Fan Yang1, C. Scarbrough1, LA. Fox2, J. Pavlik3, W. Sale2, J. Sisson3, M. Wirschell1. 1) Biochemistry, Univ Mississippi Medical Center, Jackson, MS; 2) Emory University, Dept. of Cell Biology, Atlanta, GA; 3) University of Nebraska Medical Center, Pulmonary, Critical Care, Sleep and Allergy Division, Omaha NE.

80 - 11:00
IDA6 encodes a conserved subunit required for assembly of the N-DRC and several inner arm dyneins. R. Bower1, D. Tritschler1, K. VanderWaal1, E. O'Toole2, T. Heuser3, D. Nicastro3, M. Porter1. 1) University of Minnesota, Minneapolis, MN; 2) University of Colorado, Boulder, CO; 3) Brandeis University, Waltham, MA.

81 - 11:15
CCDC39 and CCDC40 are needed for assembly of the N-DRC for motility and length control. Huawen Lin, Zhengyan Zhang, Susan Dutcher. Genetics, Washington Univ Sch Med, St Louis, MO.

82 - 11:30
Pf32p, a novel central-pair-projection protein required for flagellar beating with a regular periodicity. Takumi Wada1, Kota Abe1, Haru-aki Yanagisawa2, Toshiyuki Oda2, Takuji Nakamura1, Masahide Kikkawa2, Masafumi Hirono1. 1) Dept Biological Sci, Univ Tokyo, Tokyo, Japan; 2) Dept Cell Biology and Anatomy, Univ Tokyo, Tokyo, Japan.

83 - 11:45
Noisy rhythms of a eukaryotic flagellum. Kirsty Y. Wan, Raymond E. Goldstein. Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Wilberforce Road, Cambridge CB3 0WA, UK.


Thursday, June 12   7:15 pm–9:00 pm
Merrill Hall

Poster Awards and Announcements

Keynote Lecture

Ursula Goodenough, Washington University

From Light to Sex to Motility to Obesity: Sharing Life with Chlamydomonas and the Chlamy Community.


Ursula Goodenough will lift up highlights of her 50 years of Chlamydomonas research, starting with photosynthesis and chloroplast biogenesis studies in Paul Levine''s lab at Harvard, followed by studies of mating, flagellar architecture and signaling, cell-wall assembly, and lipid-body formation in her own lab, all with many terrific collaborators.


Presentations:

7:30
From Light to Sex to Motility to Obesity: Sharing Life with Chlamydomonas and the Chlamy Community. Ursula Goodenough. Washington University.