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2014 Important Dates


Workshop Deadline October 15
Abstract Submission Opens February 18
Conference Registration Opens February 25
Abstract Submission Deadline March 26
Chi-Bin Chien Award Nomination Deadline March 26
Abstract Revision Deadline March 27
Exhibit/ Sponsorship/ Advertising Deadline April 9
Travel Award Application Deadline May 1
Early Meeting Registration May 15
Platform and Poster Assignments online May 19
Housing Reservation May 24

Session Listing



Tuesday, June 24   6:15 PM–7:30 PM
Overture Hall

Plenary Session I: Emerging Technologies: Imaging, Genetics and Genomics

Chair: Shannon Fisher

1- 6:15
How Cells React to Noise: Direct Measurements Reveal Noise Attenuation Mechanisms in Retinoic Acid Signaling. Julian Sosnik, Likun Zheng, Enrico Gratton, Quing Nie, Thomas Schilling.

2- 6:30
Ezh2 affects the maintenance of cellular identity during embryonic development of the zebrafish. Leonie M. Kamminga, Bilge San, Nadine Wittkopp, Federico Tessadori, Simon van Heeringen, Anne Lagendijk, Emily Noël, Jeroen Bakkers, René F. Ketting.

3- 6:45
Imaging Endothelial Cell Dynamics during Vascular Network Formation. Heinz-Georg Belting, Loïc Sauteur, Anna Lenard, Alice Krudewig, Lukas Herwig, Nikolaus Ehrenfeuchter, Markus Affolter.

4- 7:00
gsx1 specifies neurons that modulate the startle response. Sadie Bergeron, Nicole Carrier, Grace Li, Harold Burgess.

5- 7:15
A map of epithelial cell dynamics guiding morphogenesis of the zebrafish fin regeneration epidermis. Chen-Hui Chen, Stefano Di Talia, Kenneth Poss.

Tuesday, June 24   7:30 PM–8:30 PM
Overture Hall Lobby

Keynote Speaker: Sarah Tishkoff

Introduction: Shannon Fisher

- 7:30
African Evolutionary Genomics: Implications for Origins, Adaptation, and Disease. Sarah Tishkoff.

Wednesday, June 25   8:30 AM–9:45 AM
Overture Hall

Neural Degeneration, Regeneration and Disease

Chair: Hiromi Hirata

6- 8:30
Mutation of zebrafish Kif5Aa tail domain causes axonal mitochondrial deficits in a mixed model of Hereditary Spastic Paraplegia/Charcot Marie Tooth Disease Type II. Philip D. Campbell, Kimberle Shen, Matthew Sapio, Thomas D. Glenn, William S. Talbot, Florence L. Marlow.

7- 8:45
Schwann cells and DCC direct regenerating motor axons onto their original path. Allison (Ali) F. Rosenberg, Roshan Jain, Clara Franzini-Armstrong, Michael Granato.

8- 9:00
Poor motoneuron development results in motor circuit defects in a vertebrate model of SMA. Christine Beattie, Hao Le, Duy Phan.

9- 9:15
Wnt signaling mediates spinal cord regeneration by radial glia. Lisa K. Briona, Richard I. Dorsky.

10- 9:30
Epidermal Cells Clear Sensory Axon Debris. Jeffrey P. Rasmussen, Georgeann Sack, Alvaro Sagasti.

Wednesday, June 25   8:30 AM–9:45 AM
Capitol Theater

Cancer and Growth Control

Chair: James Amatruda

11- 8:30
A zebrafish model for notochord-derived chordoma tumors as platform for drug screening and mechanistic studies of its tumor modifiers. Alexa Burger, Vasilyev Vasilyev, Ritu Tomar, Martin K. Selig, G. Petur Nielsen, Randall T. Peterson, Iain A. Drummond, Daniel A. Haber, Christian Mosimann.

12- 8:45
Yap Reprograms Glutamine Metabolism and Supports Growth During Liver Development and Tumorigenesis. Andrew Cox, Katie Hwang, Sebastian Beltz, Kimberley Evason, Keelin O'Connor, Evan Lien, Sagar Chhangawala, Yariv Houvras, Didier Stainier, Wolfram Goessling.

13- 9:00
Disruptions to the growth and proliferation of zebrafish intestinal mutants are mediated by distinct transcriptomes. Joan Heath, Tanya de Jong-Curtain, Karen Doggett, Janine Coates, Julia Greisbach, Christopher Love, Oliver Sieber, Aliaksei Holik, Mirana Ramialison.

14- 9:15
A scalable system for studying metastasis using zebrafish. Richard M. White, Kajan Ratnakumar, Silja Heilmann, Erin Langdon, Emily Kansler, Elizabeth Perry, Joao Xavier.

15- 9:30
Atm and Phosphohistone H2ax are Required for Synchronization of Early Cell Cycles in Vertebrate Embryos. Daniel Verduzco, Khalisa Syeda, Joshua Pierce, James Amatruda.

Wednesday, June 25   8:30 AM–9:45 AM
Playhouse Theater

Early Development and Patterning

Chair: Bruce Draper

16- 8:30
In Vitro Construction of a Vertebrate Embryo. Bernard Thisse, Peng-Fei Xu, Nathalie Houssin, Christine Thisse.

17- 8:45
Dissecting the molecular mechanism of Neural Crest Inhibition by Kctd15. V. Zarelli, I. Dawid.

18- 9:00
Dynamics and Shaping of the BMP Signaling Gradient by the BMP Antagonists During DV Axial Patterning. Joseph M. Zinski, Wei Dou, David M. Umulis, Mary C. Mullins.

19- 9:15
Wnt Promotes EMT and Mesodermal Cell Fate in the Zebrafish Tailbud via Znf703 and Cdh6. Brian Kinney, Benjamin L. Martin.

20- 9:30
Germ Cell Organizer Bucky ball Interacts via its Conserved N-Terminus with the Exosome. Stephan Riemer, Franck Bontems, Thomas Lingner, Roland Dosch.

Wednesday, June 25   8:30 AM–9:45 AM
Promenade Hall


Chair: Steve Farber

21- 8:30
Tmem18 is a regulator of adipose tissue development in zebrafish in vivo. Kathrin Landgraf, Roy Tauscher, Antje Berthold, Wieland Kiess, Antje Körner.

22- 8:45
p53-Independent Gene Regulations Are Critical for The Lysosomal Pathogenesis and Developmental Senescence in Spns1-Deficent Zebrafish. Sasaki Sasaki, Shanshan Lian, Daniel Klionsky, Shuji Kishi.

23- 9:00
Knockout Zebrafish Model for the Understanding of Prolactin Action. Yuqing Shu, Qiyong Lou, Jiangyan He, Zhan Yin.

24- 9:15
Type 2 Diabetes in a Zebrafish Model of Insulin Resistance. Lisette Maddison, Ryan Kammeyer, Kaitlin Joest, Wenbiao Chen.

25- 9:30
Diabetes gene Hnf1b is directly required for hepatic gluconeogenesis response to insulin signaling. Joseph J. Lancman, Keith P. Gates, P. Duc Dong.

Wednesday, June 25   10:45 AM–12:00 NOON
Overture Hall

Plenary Session II: Signaling and Gene Regulation

Chair: Karuna Sampath

26- 10:45
Hecate/Grip2a acts to reorganize the cytoskeleton in the symmetry-breaking event of embryonic axis induction. X. Ge, D. Grotjahn, E. Welch, J. Lyman-Gingerich, C. Holguin, E. Dimitrova, EW. Abrams, T. Gupta, FL. Marlow, T. Yabe, A. Adler, MC. Mullins, F. Pelegri.

27- 11:00
Cooperative regulation of convergence and extension by Planar Cell Polarity and notochord boundary signaling. Margot L. K. Williams, Atsushi Sawada, Chunyue Yin, Terin Budine, Lila Solnica-Krezel.

28- 11:15
Single lumen formation in the zebrafish gut is mediated by smoothened-dependent tissue remodeling. Ashley Alvers, Sean Ryan, Paul Scherz, Jan Huisken, Michel Bagnat.

29- 11:30
Rabconnectin-3a Regulates Vesicle Endocytosis and Canonical Wnt Signaling in Zebrafish Neural Crest Migration. Adam Tuttle, Trevor Hoffman, Thomas Schilling.

30- 11:45
Bmp15 is an oocyte-produced signal required for maintenance of the adult female sexual phenotype in zebrafish. D. B. Dranow, A. M. Bird, M. T. Adams, B. W. Draper.

Wednesday, June 25   1:45 PM–3:00 PM
Overture Hall

Cardiovascular Development and Disease

Chair: Jeroen Bakkers

31- 1:45
Chemokine guided angiogenesis directs coronary vasculature formation in zebrafish. Michael RM Harrison, Jeroen Bussmann, Arthela Osorio, Arndt Siekmann, Ching-ling Lien.

32- 2:00
Visnagin Protects Against Doxorubicin-Induced Cardiomyopathy Through Inhibition of Mitochondrial Malate Dehydrogenase. A. Asnani, Y. Liu, L. Zou, V. Bentley, M. Yu, Y. Wang, G. Dellaire, K. S. Sarkar, M. Dai, H. H. Chen, D. E. Sosnovik, J. T. Shin, D. A. Haber, J. N. Berman, W. Chao, R. T. Peterson.

33- 2:15
Asymmetric cell division precedes lymphatic endothelial precursor cell emergence from the cardinal vein in zebrafish. Kaska Koltowska, Anne Karine Lagendijk, Elke Ober, Alpha Yap, Benjamin M. Hogan.

34- 2:30
The role of Nkx3.2 in vascular stabilization and neural crest migration. Thomas R. Whitesell, Peter Spice, Jae-Ryeon Ryu, Sarah J. Childs.

35- 2:45
Role of FGF signaling in maintenance of cardiac chamber identity. Arjana Pradhan, Xin-Xin Zeng, Sara Marques, Neil Chi, Deborah Yelon.

Wednesday, June 25   1:45 PM–3:00 PM
Capitol Theater

Imaging 1

Chair: Sean Megason

36- 1:45
Visualization, interaction, and integrative analysis of in toto images using GoFigure2. Kishore Mosaliganti, Arnaud Gelas, Lydie Souhait, Nicolas Rannou, Nikolaus Obholzer, Sean Megason.

37- 2:00
In situ hybridization using HCR: mRNA and miRNA targets, straightforward multiplexing, high signal-to-background, sub-cellular resolution, relative quantitation. Harry Choi, Vikas Trivedi, Aneesh Acharya, Victor Beck, Scott Fraser, Niles Pierce.

38- 2:15
PyFRAP: An open-source software package for FRAP analysis. Alexander Bläßle, Patrick Müller, Ben M. Jordan.

39- 2:30
Endoderm convergence controls subduction of myocardial precusors during heart-tube formation. Fang Lin, Ding Ye.

40- 2:45
Calcium-dependent Naked-Dishevelled Interaction Modulates Wnt Signalling Outputs. AN Marsden, SW Derry, TA Westfall, DC Slusarski.

Wednesday, June 25   1:45 PM–3:00 PM
Playhouse Theater

Neural Circuits, Neurophysiology and Behavior

Chair: Stephan Neuhauss

41- 1:45
Targeted knockout of a habenula gene increases anxiety and social cohesion. Cheol-Hee Kim, Jung-Hwa Choi.

42- 2:00
Habenulo-Raphe Circuit Transmits a Danger Avoidance Signal. R. Amo, F. Fredes, M. Kinoshita, R. Aoki, H. Aizawa, M. Agetsuma, T. Aoki, T. Shiraki, H. Kakinuma, M. Matsuda, M. Yamazaki, M. Takahoko, S. Higashijima, N. Miyasaka, T. Koide, Y. Yabuki, Y. Yoshihara, H. Okamoto.

43- 2:15
Forward genetic screen reveals a role for the pregnancy associated plasma protein-a gene in habituation learning. Marc Wolman, Roshan Jain, Kurt Marsden, Hannah Bell, Katherina Hayer, John Hogenesch, Michael Granato.

44- 2:30
Postsynaptic Neurobeachin is required for electrical and chemical synaptogenesis. Adam Miller, Lisa Voelker, Arish Shah, Cecilia Moens.

45- 2:45
Genetic dissection of zebrafish circadian behaviors by TALEN and CRISPR/Cas9. Han Wang, Jian Huang, Shuqing Zhang, Yanqing Zhang.

Wednesday, June 25   1:45 PM–3:00 PM
Promenade Hall

Craniofacial and Skeletal Gene Regulation

Chair: Ferenc Mueller

46- 1:45
Tbx1 controls the morphogenesis of pharyngeal pouch epithelia through mesodermal Wnt11r and Fgf8a. Chong Pyo Choe, Gage Crump.

47- 2:00
Exploring the zebrafish periderm gene regulatory network downstream of IRF6 reveals a novel human orofacial clefting locus GRHL3. Robert A. Cornell, Myriam Peyard, Gabriel De la Garza, Jack Schleiffarth, Greg Bonde, Dan Wagner, Juha Kere, Douglas Houston, Jefferey Murray, Tiffany Smith.

48- 2:15
A Wnt-BMP signaling network balances growth and differentiation during zebrafish bone regeneration. Scott Stewart, Alan Gomez, Benjamin Armstrong, Astra Henner, Kryn Stankunas.

49- 2:30
Zebrafish mutants with craniofacial malformations reveal novel facets of suture formation. Erika Kague, Garrett Asselin, Craig Albertson, Shannon Fisher.

50- 2:45
Osteocyte-dependent and -independent osteoclast activity as a major mediator of RA-induced skull fragmentation. Shirine Jeradi, Matthias Hammerschmidt.

Wednesday, June 25   4:00 PM–6:00 PM

Recombinase-Based Genome Editing and Lineage Tracing

Room:Capitol Theater
Organizers:Tom Carney and Christian Mosimann

Recombinase systems, including Cre/lox, PhiC31, and FLP/FRT, are increasingly used for advanced transgenic experiments in zebrafish. This workshop will summarize the latest reagents, approaches, and developing uses of recombinase-based modification of the genome, including applications to transgenesis, inducible gene expression, and cell lineage tracing.

The workshop will feature presentations on both individual and large-scale resources being made available to the community and also open discussion to exchange future ideas and coordinate resources/approaches. We will further hold a FAQ section addressing common protocol problems, queries and their solutions relating to Cre/LoxP and Tamoxifen induction of Cre recombination, recombination efficiency, and transgene design.

Confirmed participants:

Jim Lister, Lister Lab, Virginia Commonwealth University
Liz Perry, White Lab, Memorial Sloan Kettering Cancer Center
Albert Pan, Pan Lab, Georgia Regents University
Le Ai Trinh, Fraser Lab, University of Southern California
Yanchao Han, Poss Lab, Duke University
Lisette Madison, Chen Lab, Vanderbilt University
Michael Parsons, Leach Lab, Johns Hopkins University

Wednesday, June 25   4:00 PM–6:00 PM

ZENCODE: Zebrafish Epigenetics

Room:Promenade Hall
Organizers:Julia Horsfield and Fiona Wardle

This workshop will highlight recent zebrafish epigenetics research, and discuss the development of a zebrafish ENCODE, or ZENCODE. A panel discussion will include working towards standardised methodology for zebrafish epigenomics so that datasets from different laboratories can be combined, or compared.

4:00 pm - 4:10 pm     Welcome and Introduction, Julia Horsfield and Fiona Wardle
4:10 pm - 4:30 pm     Brad Cairns, Epigenomic maps of zebrafish gametes and early embryos
4:30 pm - 4:45 pm     Nadine Vastenhouw, Changes in chromatin structure during genome activation
4:45 pm - 5:00 pm     Ferenc Muller, Non-canonical transcriptional and post-transcriptional regulatory mechanisms during development
5:00 pm - 5:15 pm     Antonio Girladez, Using ribosome foot printing to uncover the coding potential of the genome
5:15 pm - 5:35 pm     Derek Stemple, Functional annotation of the zebrafish genome through systematic analysis of differential gene expression
5:35 pm - 6:00 pm     Panel Discussion: integrating genomics data, genomics techniques, progressing ZENCODE

Wednesday, June 25   4:00 PM–6:00 PM

Tools for Image Acquisition, Analysis and Increased Throughput

Room:Playhouse Theater
Organizer:Rock Pulak

This workshop will discuss new tools for increasing the throughput of handling, manipulating, and orienting zebrafish larvae for image capture and some of the latest approaches for high content acquisition and analysis. The techniques discussed will cover methods for still and video images. There will be presentations on the recent advances in image acquisition and analysis in high-content screens including commercial products, such as Lightsheet microscopy (Zeiss), examples of image analysis tools (commercial and open source software), and the VAST BioImager instrumentation (Union Biometrica). Presenters will be from academic labs utilizing the increased throughput afforded by these technologies in areas such as fundamental phenotyping, drug screening and toxicology.


Karl J. Clark, Mayo Clinic, Lightsheet microscopy in toxicology studies with zebrafish larvae.
Erica Davis/Christelle Golzio, Duke University, Modeling pediatric genetic disorders in zebrafish: Accelerated throughput with VAST BioImager.
Peter Eimon, MIT, New technologies for quantitative phenotyping of zebrafish larvae.

Thursday, June 26   8:30 AM–9:45 AM
Overture Hall

Plenary Session III: Regeneration and Stem Cells

Chair: Tatjana Piotrowski

51- 8:30
Notch Signaling Enhances Self-renewal in Embryonal Rhabdomyosarcoma. Myron S. Ignatius, Eleanor Chen, Karin McCarthy, Riadh Lobbardi, Corinne Linardic, Charles Keller, David Langenau.

52- 8:45
Neuronal Signaling is Necessary for Zebrafish Appendage Regeneration. Timothy A. Petrie, Nicholas S. Strand, Aspen Gutgsell, Randall T. Moon.

53- 9:00
Imaging live Cell Dynamics of the Muscle Progenitor Cell Niche During Development. P. D. Nguyen, P. D. Currie.

54- 9:15
Spatial regionalization and heterochrony in the formation of adult pallial neural stem cells. Lara Dirian, Sonya Galant, Marion Coolen, Wenbiao Chen, Sébastien Bedu, Corinne Houart, Laure Bally-Cuif, Isabelle Foucher.

55- 9:30
Cardiac outflow tract signals control the regenerative potential of the zebrafish epicardium. Jinhu Wang, Jingli Cao, Amy L. Dickson, Kenneth D. Poss.

Thursday, June 26   10:45 AM–12:00 NOON
Overture Hall

Imaging 2

Chair: Nadine Peyrieras

56- 10:45
Non-toxic, zebrafish-optimized optogenetic gene expression system with large dynamic range and rapid kinetics. A. Reade, DY Stainier, OD Weiner, S. Woo.

57- 11:00
On the role of mechanical strains on dynamic cellular shape changes in developing zebrafish heart. Vikas Trivedi, Le Trinh, Thai Truong, Scott Fraser.

58- 11:15
Controlling apico-basal polarity within a zebrafish embryo. Clare E. Buckley, Anna Reade, Casper C. Hoogenraad, Orion D. Weiner, Jon D. W. Clarke.

59- 11:30
Zebratrack: Shining light on vertebrate behavior. Pierre Martineau, Philippe Mourrain.

60- 11:45
Genome-wide RNA tomography in the zebrafish embryo. Jan-Philipp Junker, Emily S. Noël, Eugene Berezikov, Jeroen Bakkers, Alexander van Oudenaarden.

Thursday, June 26   10:45 AM–12:00 NOON
Capitol Theater

Morphogenesis and Cell Migration

Chair: Holger Knaut

61- 10:45
Cadherin 2 Orchestrates Tissue Self-Assembly Through Two Opposing and Spatially Non-Overlapping Mechanisms. Patrick McMillen, Dörthe Jülich, Scott Holley.

62- 11:00
E-cadherin-dependent cell adhesion regulates cell migration pattern in the zebrafish lens epithelium. Toshiaki Mochizuki, Yi-Jyun Luo, Shohei Suzuki, Ichiro Masai.

63- 11:15
Epithelial flow into the optic cup facilitated by suppression of BMP drives eye morphogenesis. Stephan Heermann, Lucas Schütz, Steffen Lemke, Kerstin Krieglstein, Jochen Wittbrodt.

64- 11:30
Kremen1-mediated modulation of Wnt signaling regulates progenitor cell behavior in the lateral line. Hillary F. McGraw, Alex V. Nechiporuk.

65- 11:45
Schachbrett, a zebrafish mutant displaying disrupted stripes, encodes for Tjp1a (ZO-1.1). Andrey Fadeev, Jana Krauss, Hans-Georg Frohnhöfer, Christiane Nüsslein-Volhard.

Thursday, June 26   10:45 AM–12:00 NOON
Playhouse Theater

Chemical Biology

Chair: Elizabeth Patton

66- 10:45
Modeling and preventing anthracycline and tyrosine kinase inhibitor induced cardiotoxicity in Zebrafish. Victoria L. Bentley, Chansey J. Veinotte, Yan Liu, Randall T. Peterson, Graham Dellaire, Jason N. Berman.

67- 11:00
Novel Renal Progenitor Chemical Screen Identifies Prostaglandins as Modulators of Proximo-Distal Segment Fate Choice During Zebrafish Pronephros Development. Shahram J. Poureetezadi, Eric K. Donahue, Rebecca A. Wingert.

68- 11:15
Chemical Genetic Screening in Zebrafish Identifies Nitric Oxide Signal and Histone Acetylation to Coordinate Neural Crest Development and Craniofacial Morphogenesis. Yawei Kong, Michael Grimaldi, Eugene Curtin, Max Dougherty, Charles Kaufman, Richard White, Leonard Zon, Eric Liao.

69- 11:30
Behavior-based screening identifies compounds controlling the switch between passive and active threat responses. A. J. Rennekamp, S. Patel, P. J. Lorello, X. P. Huang, L. Cade, B. L. Roth, B. J. Caldarone, D. Kokel, R. T. Peterson.

70- 11:45
Systematic phenotype prediction to accelerate reverse genetic and small-molecule screening. Gabriel Musso, Murat Tasan, Frederick Roth, Calum MacRae.

Thursday, June 26   10:45 AM–12:00 NOON
Promenade Hall


Chair: David Parichy

71- 10:45
Adaptive changes in vibration attraction behavior and its sensory receptors promote eye degeneration in the cavefish, Astyanax mexicanus. Masato Yoshizawa, Kelly O'Quin, Alex Keene, William Jeffery.

72- 11:00
Pigment cell heterochronies underlying pattern evolution in Danio fishes. Larissa Patterson, Emily Bain, David Parichy.

73- 11:15
Phylo-Mapping: a comparative genomic approach to understand natural variation. Jacob Daane, Matthew Harris.

74- 11:30
Resolution of the Danio Phylogeny through RAD Phylogenomics. Braedan M. McCluskey, Peter Batzel, John H. Postlethwait.

75- 11:45
The evolution of morphological novelty through the EDA-mediated regionalization of skin appendages. M. Brent Hawkins, Alexandra Rodriquez, Katrin Henke, Sven Reischauer, Matthew P. Harris, David W. Stock.

Thursday, June 26   1:30 PM–2:30 PM
Overture Hall

Keynote Speaker: John Postlethwait

Introduction: Tom Schilling

- 1:30
Connecting teleost genomes to human biology. John Postlethwait.

Thursday, June 26   2:30 PM–3:00 PM
Overture Hall

Chi-Bin Chien Award Presentation
Introduction: David Grunwald

- 2:30
From genomics to novel gene functions in zebrafish. Andrea Pauli.

Thursday, June 26   4:00 PM–6:00 PM

Emerging Tools and Methods for Quantitative Imaging of Vascular Development

Room:Playhouse Theater
Organizers:Ben Hogan and Shane Herbert

This workshop will highlight new approaches in the analysis of the zebrafish vasculature with a particular focus on the visualization and quantification of dynamic cellular processes during vascular development. Emphasis will be placed on utilization of advanced real-time imaging approaches that push the current boundaries of cellular and temporal resolution. In particular, there will be a focus on the integration of imaging studies with computational modeling and novel analytical methods to provide a quantitative interpretation of vessel phenotypes. In addition, there will be discussion from speakers about the commonly used tools in the field. These tools, as well as the emerging techniques discussed above, all promise to be broadly applicable in vascular cell biological studies in zebrafish.

Topics include: Advanced real-time imaging techniques, New analytical tools and computational methods for the quantification of angiogenic phenotypes, Sub-cellular resolution imaging of vascular organelle dynamics, achieving phenotypic specificity in analysis of vascular phenotypes.

Confirmed Speakers:

Heinz-Georg Belting (Affolter Lab), University of Basel, Switzerland

Emily Steed (Vermot Lab), IGBMC, France

Katie Bentley, Harvard University, USA

Nathan Lawson, University of Massachusetts, USA

Shane Herbert, University of Manchester, UK

Thursday, June 26   4:00 PM–6:00 PM

Practical Approaches to Using Zebrafish in Education

Room:Wisconsin Studio
Organizers:Jennifer Bonner and Suzanne Gribble

Using zebrafish for educational purposes? Our time will be spent engaging in large and small group discussions on the topics of teaching students to read scientific literature, outreach program development, and how to lead undergraduate research students through the process of designing, implementing, analyzing and presenting research projects while accomplishing your teaching and research objectives.

Confirmed participants:

Nathalia Holtzman, Queens University of New York City
Steven Farber, Carnegie Institute for Science

Thursday, June 26   4:00 PM–6:00 PM

Post-embryonic development: expanding human disease models and understanding variation

Room:Promenade Hall
Organizer:Matthew Harris

Zebrafish is quickly becoming the ‘go to’ model for analysis of gene function during development and in assessing genetic variants associated with human disease. Post-embryonic development is becoming an important focus of research as beginning studies point to the utility of late phenotypes in disease modeling as well as to understand variation in form and physiology. However, there remains a lack of a unified discussion of the questions/frontiers in the field and what tools are needed to take advantage of the zebrafish to address these questions.

The workshop is designed to bring together researchers that have focused on mechanisms of late development to discuss important aspects of organogenesis and physiology that are uncovering novel aspects of gene function and providing important models for disease. Topics will center on particular organ systems or processes to highlight the current state of the field, where the field is going, as well as developing specific tools for analysis. The overall goal of the workshop is to establish common goals and efforts to promote use of adult zebrafish in modeling disease and understanding variation among vertebrates.

Confirmed speakers:

Development of Skeleton:
Matthew Harris, Harvard Medical School; Boston Children’s Hospital
Shannon Fisher, University of Pennsylvania

Adipogenesis and Gut Microbial dynamics:
John Rawls, Duke University

Adult neurogenesis and behavior:
Hitoshi Okamoto, RIKEN Brain Science Institute

Kellee Siegfried, University of Massachusetts Boston
Bruce Draper, University of California Davis

Life History Regulation: Metamorphosis:
David Parichy, University of Washington

Aging and senescence:
Matthew Harris, Harvard Medical School; Boston Children’s Hospital

Friday, June 27   8:30 AM–9:45 AM
Overture Hall

Gene Regulation and Epigenetics

Chair: Antonio Giraldez

76- 8:30
Transcriptional control mediated by cohesin and Ctcf in early zebrafish development. Michael Meier, Jenny Rhodes, Justin M. O'Sullivan, Julia A. Horsfield.

77- 8:45
Cardiomyocyte-specific profiling of gene-regulatory programs during heart regeneration using the replacement histone H3.3. Joseph A. Goldman, Amy L. Dickson, Jaclyn M. Karasik, Sean Thomas, Benoit G. Bruneau, Kenneth D. Poss.

78- 9:00
The capture and analysis of cardiac lineage-specific and stage-specific gene expression profiles in zebrafish. Todd A. Townsend, Bushra Gorsi, H. Joseph Yost.

79- 9:15
Analyzing multigenerational effects of the Piwi/piRNA pathway in zebrafish. Holger Dill, Rene' Ketting.

80- 9:30
Single cell transcript counting. Steven Harvey, John Collins, Neha Wali, Ian Sealy, Jorge Zamora, Ian Packham, Derek Stemple.

Friday, June 27   8:30 AM–9:45 AM
Capitol Theater

Neural Development

Chair: Rich Dorsky

81- 8:30
Serotonin Regulates Midline Axon Crossing via EphrinB2. Lingyan Xing, Jong-hyun Son, Tamara Stevenson, Tiffanie Dahl, Josh Bonkowsky.

82- 8:45
The adhesion GPCR Gpr126 has distinct, domain-dependent functions in Schwann cells governed by interaction with Laminin-211. Sarah C. Petersen, Rong Luo, Amit Mogha, Torsten Schöneberg, Ines Liebscher, Xianhua Piao, Kelly R. Monk.

83- 9:00
Calsyntenin-1 Regulates Axon Branching and Endosomal Trafficking During Sensory Neuron Development in vivo. O. Y. Ponomareva, I. C. Holmen, A. J. Sperry, K. W. Eliceiri, M. C. Halloran.

84- 9:15
Anterograde axonal transport involvement in synaptic function and growth coordination. Thomas O. Auer, Tong Xiao, Valerie Bercier, Christoph Gebhardt, Karine Duroure, Jochen Wittbrodt, Herwig Baier, Filippo Del Bene.

85- 9:30
Regulation of dynein-mediated mitochondrial transport in axons. Catherine Drerup, Alex Nechiporuk.

Friday, June 27   8:30 AM–9:45 AM
Playhouse Theater

Cilia, Cell and Tissue Polarity

Chair: Rebecca Burdine

86- 8:30
The Role of Planar Cell Polarity in Directed Cell Migration. Andrew W. Mathewson, Crystal Davey, Cecilia Moens.

87- 8:45
Intraciliary calcium waves initiate vertebrate left-right asymmetry. Shiaulou Yuan, Lu Zhao, Martina Brueckner, Zhaoxia Sun.

88- 9:00
Prostaglandin signaling regulates ciliogenesis by modulating intraflagellar transport. Daqing Jin, Terri T. Ni, Jianjian Sun, Guangju Yu, Haiyan Wan, Jeffrey D. Amack, Jonathan Fleming, Chin Chiang, Satish Cheepala, Gwenaëlle Conseil, Susan P. C. Cole, Iain Drummond, John D. Schuetz, Jarema Malicki, Tao P. Zhong.

89- 9:15
BBS chaperonin complex function in zebrafish transport and vision. Charles Scott, Denise Oh, Daniel Grigsby, Trudi Westfall, Xitiz Chamling, Diane Slusarski.

90- 9:30
Hipk2 and PP1c-mediated dephosphorylation of Dishevelled sustains Wnt signal transduction. Nobuyuki Shimizu, Shizuka Ishitani, Tohru Ishitani.

Friday, June 27   8:30 AM–9:45 AM
Promenade Hall

Infection and Immunity

Chair: David Tobin

91- 8:30
Regulators of host defense are critical drivers of macrophage hematopoiesis. Celia E. Shiau, William S. Talbot.

92- 8:45
Immune compromised zebrafish as a platform for cell transplantation. John C. Moore, Qin Tang, Nouran S. Abdelfattah, Jessica S. Blackburn, Sarah A. Martinez, Finola E. Moore, Riadh Lobbardi, Myron S. Ignatius, David M. Langenau.

93- 9:00
Cxcr3/Cxcl11 Signaling Mediates Macrophage Activation and Chemoattraction to Bacterial Infection in Zebrafish. Vincenzo Torraca, Chao Cui, Ralf Boland, Jan-Paul Bebelman, Marco Siderius, Martine J. Smit, Astrid M. van der Sar, Herman P. Spaink, Annemarie H. Meijer.

94- 9:15
Dose-dependent virulence changes following localized infection in zebrafish embryos with a Listeria monocytogenes strain designed to hyper-activate the Nlrc4 inflammasome. William JB Vincent, John-Demian Sauer, Anna Huttenlocher.

95- 9:30
Epigenetics Regulation of Intestinal Inflammation in Zebrafish. Lindsay Marjoram, Ashley Alvers, Elizabeth Deerhake, Jennifer Bagwell, Jamie Mankiewicz, Rebecca Beerman, Jason Willer, Nicholas Katsanis, David Tobin, John Rawls, Mary Goll, Michel Bagnat.

Friday, June 27   10:45 AM–12:00 NOON
Overture Hall

Plenary Session IV: Physiology and Disease

Chair: Graham Lieschke

96- 10:45
Chemical screen shows link between β-catenin and the c-Jun-N-terminal kinase pathway in liver tumor formation. Kimberley Evason, Andrei Goga, Didier Stainier.

97- 11:00
Antioxidant Treatment Rescues Hematopoietic Phenotypes in Different Models of Adenylate Kinase 2 Deficiency. Alberto Rissone, Katja Weinacht, Jaya Jagadeesh, Karen Simon, Kevin Bishop, Raman Sood, Luigi Notarangelo, Fabio Candotti.

98- 11:15
A chemical suppressor of tif1γ mutant moonshine reveals that PPARα bypasses the transcriptional pausing defect in erythroid differentiation. Marlies P. Rossmann, Xiaoying Bai, Bilguujin Dorjsuren, Lauren Krug, Chang-Ying Chiang, Erika Lee, Wahaj Chaudhry, Shakkaura Kemet, Isaac Adatto, Leonard I. Zon.

99- 11:30
Interception of host angiogenic signalling limits mycobacterial growth. Stefan H. Oehlers, Ninecia R. Scott, Monica I. Thomas, Kazuhide S. Okuda, Mark R. Cronan, Philip S. Crosier, David M. Tobin.

100- 11:45
Genetic analysis of the acoustic startle behavioral threshold. Kurt Marsden, Roshan Jain, Marc Wolman, Hannah Bell, Lauren Schmidt, Julianne Skinner, Katharina Hayer, John Hogenesch, Michael Granato.

Friday, June 27   2:00 PM–4:00 PM

Cancer in the Zebrafish: moving from promise to achievement

Room:Overture Hall
Organizers:Jason Berman and David Langenau, Richard White

The zebrafish has emerged as a powerful model for human disease over the past decade, but still remains at the periphery of most mainstream disease studies, particularly in cancer. The purpose of this workshop is to give an overview of several major technologies available in the zebrafish (both strengths and limitations), and how these present unique opportunities to address problems in cancer biology. In addition to the talks, we will leave time for a discussion of strategies to propel the zebrafish into a prominent place in cancer modeling.

Rich White, A brief introduction to cancer modeling in the zebrafish
Rich White, Genomics Approaches (DNA/RNAseq)
Yariv Houvras, Epigenetics
Jane Zhu, Functional Genomics
Joanna Yeh, Chemical Genetics
David Langenau, Allograft Transplantation
Jason Berman and Herman Spaink, Xenograft Transplantation

Friday, June 27   2:00 PM–4:00 PM

Emerging Techniques in Neural Circuit Analysis

Room:Capitol Theater
Organizers:Filippo del Bene and Marnie Halpern

With recent progress in live brain imaging, the zebrafish is a superior model for correlating neural activity with behavior. The workshop "Emerging techniques in neural circuit analysis" will present new technologies for the analysis of neural circuit development and function. Participants will discuss potential problems and successful attempts to apply new transgenic tools such as genetically encoded voltage and calcium indicators, optogenetic probes, brainbow and transynaptic labeling, and novel behavioral paradigms. The goal is to coordinate the efforts of multiple labs in adapting state-of-the-art approaches to zebrafish, by highlighting recent advances and addressing unresolved challenges.

Speaker list:

Erik Duboué, Carnegie, Quantifying the response to an aversive stimulus in larval zebrafish

Claire Wyart, ICM, France, Channel Rhodopsin mediated mapping of connectivity in the spinal cord of zebrafish larva

Douglas Kim, Janelia, Optimized fluorescent proteins for imaging neural activity

Hitoshi Okamoto, Riken, Japan, Optogenetics in adult zebrafish

Koichi Kawakami, NIG, Japan, Genetic dissection of the adult zebrafish brain by the Gal4-UAS method

Misha Ahrens, Janelia, Whole-brain functional maps

Kurt Marsden, Univ. of Penn, Mapping neural circuits underlying startle modulation in head restrained zebrafish larvae

David Prober, Caltech, Using TRP channels to activate and ablate neurons

Alex Schier, Harvard, An immunohistochemical method to detect neuronal activity

Harold Burgess, NIH, New transgenic tools for manipulating neuronal circuitry

Friday, June 27   2:00 PM–4:00 PM

Maternally Controlled Development - Current Limitations of Analysis and Future Perspectives

Room:Promenade Hall
Organizers:Sreelaja Nair and Roland Dosch

Early development of all eukaryotic embryos relies on factors loaded into the egg by the female prior to fertilization. Dissecting their roles poses several technical challenges primarily in the isolation of critical maternal factors, the visualization of their dynamic cellular localization and analyzing their biochemical function. Although zebrafish emerged as an outstanding vertebrate for the analysis of maternal factors, our understanding of their function is mostly limited to forward genetic mutation approaches. The workshop is intended to share through short talks, innovations under development to broaden the methodologies that can be used to effectively study maternal genes. The goal of the subsequent discussion is to elaborate on efforts to circumvent technical limitations and resource sharing so that zebrafish continues to excel in all aspects of research on maternally controlled development.

Short Talks

Francisco Pelegri, Genetic approaches to isolate maternal genes

Roland Dosch, Protein knockdown to investigate maternal factors

Sreelaja Nair, Oocyte culture methods and targeted isolation of maternal factors

Karuna Sampath, Packing and moving: localized transport in oocytes and early embryos

Friday, June 27   2:00 PM–4:00 PM

Digestive System Biology and Disease

Room:Wisconsin Studio
Organizers:Wolfram Goessling and John Rawls

This workshop will bring together the growing community of researchers using the zebrafish to investigate digestive organ development, physiology, and disease. The format of this workshop will consist of a series of short presentations and community discussions about emerging technologies, resources, needs, funding strategies, and other topics of specific relevance to this community.

2:00 pm       Michel Bagnat, Duke University, Genetic and cell biological approaches to study digestive tract development and function in zebrafish

2:25 pm       Andy Cox, Brigham and Women''s Hospital, ,Zebrafish models of liver injury and their possible translational application

2:50 pm       Stephen Watts, University of Alabama at Birmingham, Zebrafish nutrition: opportunities lost

3:15 pm       John Rawls, Duke University, Factors influencing microbiota assembly in the zebrafish intestine

3:40 pm       Steven Farber, Carnegie Institution, Involvement of the digestive system research community in Zebrafish Disease Models

Saturday, June 28   9:00 AM–10:15 AM
Overture Hall

Genetics and Genomics

Chair: Brad Cairns

101- 9:00
High-throughput Targeted Mutagenesis using CRISPR-Cas9 in Zebrafish. Gaurav K. Varshney, Wuhong Pei, Johan Ledin, Matthew LaFave, Jennifer Idol, Viviana Gallardo, Lisha Xu, Marypat Jones, Ursula Harper, Blake Carrington, Kevin Bishop, Meghana Vemulapalli, Mingyu Li, Wenbiao Chen, James Mullikin, Raman Sood, Shawn M. Burgess.

102- 9:15
GAPmap: Small Meiotic Linkage Groups to Fix Gaps in the Zebrafish Reference Genome Assembly. Derek L. Stemple, Ian Sealy, Ross N. W. Kettleborough, James Torrance, Kerstin Howe.

103- 9:30
A simple method for targeted insertions into zebrafish using TALENs. Sean Constable, Sebastian Gerety, David Wilkinson.

104- 9:45
Advances in Gene-Targeting in the Zebrafish. Kazuyuki Hoshijima, Mick Jurynec, David Grunwald.

105- 10:00
The Zebrafish Mutation Project: New Mutagenesis and Phenotyping Strategies. Elisabeth M. Busch-Nentwich, John Collins, Neha Wali, Ian Sealy, Peter Clarke, Christopher M. Dooley, Catherine Scahill, Richard J. White, Nicole Staudt, Samantha Carruthers, Jorge Zamora, Zsofia Pusztai, Isabel Brocal, Amanda Hall, Richard Clark, Richard Gibbons, Ross N. W. Kettleborough, Derek L. Stemple.

Saturday, June 28   9:00 AM–10:15 AM
Capitol Theater


Chair: Zilong Wen

106- 9:00
Epigenetic therapy restores normal hematopoiesis in a zebrafish model of NUP98-HOXA9-induced myeloid disease. Adam Deveau, A. Micheal Forrester, Andrew Coombs, Gretchen Wagner, Clemens Grabher, Ian Chute, Daniel Leger, Stephen Lewis, A. Thomas Look, Jason N. Berman.

107- 9:15
Mutation of kri1l causes ribosomopathy-like hematopoiesis failure via excessive autophagy. Xiaoe Jia, Ke Ma, Chunguang Ren, Changbin Jing, Cong Fu, Wenjuan Zhang, Shuang Wu, Yi Chen, Yi Jin, Mei Dong, Min Deng, Lingfei Luo, Li Li, Yi Zhou, Leonard Zon, Saijuan Chen, Weijun Pan.

108- 9:30
FGF signaling specifies hematopoietic stem cells through its regulation of somitic Notch signaling. Y. Lee, J. Manegold, A. Kim, D. Stachura, W. Clements, D. Traver.

109- 9:45
Role of tp53 in the oxidative stress response of erythroid precursors. Michelle Carter, Richard Shimshock, Ashley Kramer, Troy Lund.

110- 10:00
Advantages of Clonal Zebrafish for Transplantation Assays and Investigation of the Immune System. Sean C. McConnell, Wilfredo Marin, Michael Peters, Jill L. O. de Jong.

Saturday, June 28   9:00 AM–10:15 AM
Promenade Hall

Sensory Systems

Chair: Teresa Nicolson

111- 9:00
Characterizing the Roles of Sox2 and Sox3 in Sensory/Neural Patterning During Zebrafish Inner Ear Development. Yunzi Gou, Hye-Joo Kwon, Bruce Riley.

112- 9:15
Neurogenin1 regulates the projection of axons of olfactory neurons via its target cxcr4b. Romain Madelaine, Raphael Aguillon, Laurence Garric, Caroline Halluin, Pascale Dufourcq, Patrick Blader.

113- 9:30
Assaying retinal circuit function and vision in the retina lacking Müller glia cells. Ryan MacDonald, Nachiket Kashakar, Jingjing Zang, Stephan Neuhauss, Leon Lagnado, William A. Harris.

114- 9:45
Exploring the roles of Transmembrane channel-like proteins in mechanotransduction in zebrafish hair cells. Reo Maeda, Weike Mo, Katie Kindt, Timothy Erickson, Clive P. Morgan, Adam Therneau, Rachel Clemens-Grisham, Peter G. Barr-Gillespie, Teresa Nicolson.

115- 10:00
In toto imaging of the developing endolymphatic duct and sac reveals cycles of tissue inflation and deflation. Ian A. Swinburne, Ebaa F. Al-Obeidi, Sean G. Megason.

Saturday, June 28   9:00 AM–10:15 AM
Wisconsin Studio

Endodermal and Mesodermal Organs

Chair: Joan Heath

116- 9:00
Sdf1-expression Reveals a Source of Perivascular-Derived Mesenchymal Stem Cells in Zebrafish. Michelle Carter, Nardina Nash, Paul Stadem, Ashley Kramer, Troy Lund.

117- 9:15
Genetic Analysis and Characterization of the Novel Zebrafish Podocyte Mutant zeppelin. Paul T. Kroeger, Rachel Miceli, Michael McKernan, Annemarie Fox, Rachel Bounds, Jennifer Cihlar, Ignaty Leshchiner, Alan J. Davidson, Wolfram Goessling, Rebecca A. Wingert.

118- 9:30
Transcriptome profiling of adult zebrafish centroacinar cells reveals their similarity to larval endocrine pancreas progenitors. R. L. Beer, G. Wang, F. Delaspre, W. Huang, S. Gee, M. Rovira, M. Bagnat, S. Wheelan, M. J. Parsons.

119- 9:45
Pseudophosphatase dusp27 is a novel sarcomere protein and required for the development of the skeletal muscle contractile apparatus. Eric Horstick, Kandice Fero, Sadie Bergeron, Hiba Codore, Grace Li, Fumihito Ono, James Dowling, Harold Burgess.

120- 10:00
Elucidating the Mechanisms of Therapeutic Augmentation of Kidney Repair After Acute Kidney Injury. Lauren Brilli, Takuto Chiba, Nataliya Skrypnyk, Lee Mcdermott, Mark De Caestecker, Neil Hukriede.

Saturday, June 28   10:45 AM–12:00 NOON
Overture Hall

Plenary Session V: Morphogenesis and Organogenesis

Chair: Tom Schilling

121- 10:45
Phosphoinositide recycling and angiogenesis. Amber M. Stratman, Coinstantinos Mikelis, Pezoa Sofia, J. Solivo Gutkind, Brant M. Weinstein.

122- 11:00
Mutations in ext genes reveal that Heparan Sulfate Proteoglycans regulate Wnt/β-catenin and Fgf signaling domains in the lateral line primordium. Marina Venero Galanternik, Kenneth Kramer, Tatjana Piotrowski.

123- 11:15
The Iroquois transcription factors Irx7 and Irx5a promote the zebrafish hyoid joint by arresting chondrocytes at an early state of maturation. Amjad Askary, Xinjun He, Lindsey Mork, Shinsuke Ohba, Andrew P. McMahon, Gage Crump.

124- 11:30
Cellular asymmetry in angiogenesis revealed by live imaging in zebrafish. Donna J. Page, Didier Y. Stainier, Shane P. Herbert.

125- 11:45
Motor exit point (MEP) glia: Novel CNS-derived glia that segregate myelinating glia at the MEP. Sarah Kucenas, Cody Smith, Angela Morris, Taylor Welsh.